Abstract: Amylase in the body is used to break down polysaccharides into monosaccharides. The monosaccharides are simple sugars that are used to give the body energy. This effect was investigated in an experiment designed to show the breaking down of starch solution. A water bath was used to heat water up to 60oc. Pieces of dialysis tubing were wet and had starch solution put inside.…
These solutions include glucose, maltose, honey, lactose and beer. There was precipitation formed in these solutions, which resulted in the colour change indicating that sugar must be present in these solutions for this to occur (Nigam et.al, 2003). Monosaccharides such as glucose, maltose and lactose are all defined as simple sugars and since they showed a positive result in this test, it indicates that the Benedict’s test was performed correctly (Alberts et. al, 2014).…
The test was determined complete when sample of the reaction mixture was repeatedly added to the potassium iodide starch test. Once the paper no longer turns blue-black the reaction is considered complete. Potassium iodide starch test paper is a paper strip use to determine if excess sodium hypochlorite remains in the flask. A diethyl extraction was used to remove the lower aqueous layer of the product after the potassium iodide starch test. This removes unreacted diol and leftover acids.…
This same procedure was reiterated with the fungal amylase. Samples were obtained and placed in spaces on a clean spot plate where each…
The component of starch which causes the colour change is amylose. The iodine molecule slides inside the amylose coil. However, iodine is insoluble in water so it is added to potassium, creating the the iodine reagent which dissolves in amylose causing the cloudy white colour to become black.(5). The Iodine in the ECF was able…
Being a activity unit for enzyme, the unit (SKB/g) is defined as 1 g substrate (starch) can be hydrolyzed by 1 g α-amylase within 1 hour. Sternzym VC 5000 is extensively utilized in food industry owning to its applicability almost in all types of yeast-raised goods like bread, rolls…
The starch hydrolysis test was observed to be negative. However, brown stated that the test for Bacillus subtilis should come out positive. When the starch plate was observed, there was a lot of growth, and there was only a small circle that was hydrolyzed in the middle of the plate.…
The iodine gets stuck in the coils of beta amylose molecules and results in the mixture. There is some transfer of charge between the starch and the iodine. That changes the way electrons are confined, and so, changes the arrangement of the energy levels. These new energy level arrangements absorb visible light- giving the compound its intense bluish/purple color (How does starch indicate iodine, 2013). If iodine is, however, mixed with a compound that doesn’t contain starch, there would be no visible…
Each compound reacted with one reactant; the glucose reacted with the Benedict’s solution, the Starch reacted with the Iodine, and the Protein reacted with the Biuret’s solution. In each test the compounds and the water all changed when mixed with the reactants and took on a lighter shade of the reactant’s color. The reaction that stood out the most was between the glucose and the Benedict’s solution. The glucose and Benedict’s solution took much longer while the other reactions seemed to be nearly the instant. The change in color was extremely different as well, while the other reactions seemed to be similar to the color of the reactant, the reaction between the glucose and the Benedict’s was almost the opposite from reactant’s original color.…
The study of the “impact of artificial light on the production of sugars and starch by seedlings” was conducted to compare the effectiveness of seed artificial light compared to natural light to see if artificial light could be used as a substitute for artificial light. To produce the results to create a comparison, students conducted Starch Testing and Carbohydrate testing. The starch testing consisted 6 tubes, two of which were controlled samples with and without a Lugol’s Iodine, two experimental samples with and without Lugol’s, Iodine, as well as one tube with water that contains Lugol’s Iodine. Each tube that contains Lugol’s Iodine had three drops of Lugol’s and ten drops of the other solution.…
Through this experiment we measured how fast a chemical reaction occurs, by changing the degradation rate in Albumin when added to different enzymes. Albumin is a protein found in egg white, which is considered to have important storage and nutritional functions. Albumin has also been used in medicine to treat heavy metal intoxication. We ran Albumin through four different conditions to see which would make the Albumin degrade faster. We predicted that the Albumin would degrade with pepsin the fastest since it is essential for digestion of substances in the stomach.…
2.4 Amylase’s Role in Bread Processing Bread making process is different from one company and the other company. There are many uniqueness of formula from each company but generally, the process is divided into three processes. The first step is dough mixing. The starch inside the flour can be broken by the alpha-amylase and will produce dissolved amylose which is the enzyme substrate to the next amylose degradation process. Starch hydrolysis process has a very important role to dough’s rheology characteristic because amount of water will be bonded by the hydrolyzed starch.…
Introduction: Enzymes have a huge effect on the human body and other organisms. Enzymes are catalysts that are used to accelerate the reaction process by lowering the activation energy. For this experiment the catechol oxidase was extracted from potatoes. The enzyme is called catechol oxidase because the oxygen is reacting to catechol. Catechol oxidase causes the browning of the fruits and vegetables because the compound is toxic to the bacteria.…
Explain the results you obtained using a piece of muscle and a piece of potato?. Both the test tubes will show decomposition of hydrogen peroxide because both potato and uncooked ham have catalase enzymes. 5. What effect did acetic acid (vinegar) have on the reaction?…
I tested the mixed solution for starch by removing a small drop of the reaction mixture with a clean eye dropper and added it to the first drop of iodine in the spotted white tile. 12. I noted the colour. 13. I discarded the mixture in the eye dropper and got a new drop from the test tube.…