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7 Cards in this Set
- Front
- Back
Protein separation technique based on size and/or charge depending on conditions.
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Electrophoresis
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Protein separation technique by charge- pH gradient established in a gel matrix, protein travels to pI. This technique can separate proteins with v. similar pIs.
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Isoelectric focusing.
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Protein separation technique based on size only by first giving all amino acids similar charge-to-mass ratios.
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SDS-PAGE
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Which protein separation technique combines isoelectric focusing and SDS-Page
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2D gels- can separate based on size and charge
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Name three characteristics of the peptide bond found between amino acids in proteins.
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1. The carbonyl oxygen and amide hydrogen are in the trans configuration
2. Has two permanent dipoles aligned in the same direction 3. Has partial double bond character and thus cannot rotate |
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Which amino acid is considered a helix breaking or kinking amino acid?
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Proline
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Hydrophobic collapse is the driving force in what level of protein folding?
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Tertiary
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