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    Cohesion Fatigue Theory

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    overlooked source, cohesion fatigue can induce chromosome instability. The authors focused on the cohesion complex in this study since in a previous study they identified that a certain protein, Ska3, was important in the maintaining of chromosome cohesion2. This implies that there is a possibility of other proteins that can destabilize chromosome cohesion, thus creating chromosome instability. 2. Cohesion fatigue is a phenomenon named by the authors, in which during induced metaphase delay,…

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    Biocon India Case Study

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    • What are the strategic choices available? Biocon India Group is a Bangalore-based organization that began in 1978 with the enzyme business. An enzyme, by the way, is a substance produced by a living organism which acts as a catalyst to bring about a specific biochemical reaction. That said, the Biocon India realized that growth is imminent and various strategic choices are available. The first strategic choice available to Managing Director, Kiran Mazumdar-Shaw’s Biocon India is expansion…

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    Five Nutrients

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    Introduction: There are five main nutrients in food: 1. Protein 2. Carbohydrates 3. Lipids 4. Minerals 5. Vitamins Nutrients are part of a nutritious diet. It is important when trying to live a healthy lifestyle to get the correct balance of nutrients to say healthy. Nutrients are needed to build up and repair body tissue and cells that may become damaged or weakened. They help to maintain our vital organs and help keep our bones in working condition which helps strengthen us. Nutrients help…

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    2. What are the differences between deep freezer storage and cryo-storage methods? Deep freezing storage and cryo-storage, methods are some of the methods frequently used to preserve microorganisms for a long term storage. Both methods use extremely low temperature to ensure the biological material remains genetically stable and metabolically inert. However both methods differ slightly from each other. Cryo-storage, also known as cryopreservation, uses liquid nitrogen to freeze the…

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    OMEGA-3 FATTY ACIDS 1) Structure Figure 1.1 The omega-3 fatty acids are also known as ω-3 fatty acids or n-3 fatty acids which contain a long chain of polyunsaturated fatty acids and the chain ranges from 18 to 22 carbon atoms (Collins J.J., 2010). The fatty acids have two ends; the carboxylic acid end (-COOH) and the methyl end (-CH3). The carboxylic acid end is considered the starting of chain which is “alpha” and methyl chain is considered as the tail of the long chain…

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    Leroy et al. (2000) used 4 microsatellite primers to characterise Brassica oleracea accessions. Among the 136 reproducible fragments generated, 25 (18.4%) fragments were common for all Brassica, 27 (19.9%) were unique and 84 (61.7%) were phylogenetically informative. Flannery et al. (2006) assessed polymorphisms in Brassica, Arabidopsis, Camelina, Raphanus and Sinapis using 10 plastid SSR primer sets. Eight loci were polymorphic, and separated the individuals of Brassicaceae into taxon-specific…

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    act the same, some directly bind to amino acids, while others relay the signals indirectly through protein interactions. Identifying FLCN amino acid reservoir allows us to determine how FLCN interacts with amino acids by providing insight whether the interaction is direct or…

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    Phase 2 – Agarose Gel Electrophoresis 1. Obtain our four H, E, L, and P labeled micro tubes and place them on ice. 2. Set the micro pipet to 2.0 ul. 3. Transfer 2.0 ul blue dye to each of the four H, E, L, and P micro tubes. 4. Mix the four micro tubes by using this two-step method: * Place each tube briefly on the vortex to get the components to collect at the bottom of the micro tubes * Pulse spin the four micro tubes in the centrifuge to mix all of the components completely. 5.…

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    Potato Dextrose Agar Essay

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    4.1 Maintenance and sub-culturing of endophytic fungi The cultures were procured from already maintained repository in laboratory. The maintenance of the cultures involved preparation of Potato dextrose agar (PDA) plates, sub culturing of cultures and long term preservation. 4.1.1 Preparation of PDA (Potato dextrose agar) plates 39g of PDA was dissolved in 1000 ml double distilled water, stirred to mix properly and was transferred into 250 ml Erlenmeyer flasks and autoclaved at 121˚C at 15…

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    1.4 Effect of Microcrystalline Cellulose (MCC)Type Formula F12 was utilized to study the effect of changing the type of MCC(Avecil PH-101 ) as a carrier material instead of (Avecil PH-102) in F8 on the dissolution behavior of the prepared Zolmitriptan liquisolid ODTs. Although the DT of formula F12 (79 sec.) was greater than F8 (48 sec.) as in Table(18),the dissolution behavior of the formula F12 was faster than F8 as in Table (18) and Figure (48), this difference was non…

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