are phylogenetically subdivided into two groups, type I and type II. The main difference between them is that type I NPVs contain the fusion protein GP64, needed for virus entry into the cell and for cell-to-cell transmission, while type II NPVs lack GP64 and instead contain a generic fusion protein, called F protein, with the same role as GP64. This protein can also be found in some vertebrate viruses (Szewczyk et al.,…
Title: How Temperature Affects Protein Detection In Eggs Whites Introduction: Research Objective and Hypothesis The objective of this experiment is to gain a better understanding on how temperature affects how much protein is detected. Understanding how temperature affects the detection of protein can be used to more accurately measure the amount of protein in foods and supplements needed for life. This knowledge can benefit nutritionists and the general population. Hypothesis: If the egg…
Ca2+/calmodulin-dependent protein kinases, calcineurin and phosphorylase kinase. The Ca2+ sensitive and potassium channels are potassium channels either in the nucleus or cytoplasm which open and close in response to Ca2+. This channel regulates transmitter release, muscular contraction, excitation of neurons, up-regulation of gene expression, release of hormones and neurotransmitters (FABER and SAH, 2016). The Ca2+ calmodulin sensor activates effector Ca2+/calmodulin-dependent protein…
production of the protein menin that acts as a tumor suppressor. It prevents cells from growing and dividing too rapidly. Mutations of the MEN1 gene result in an altered menin protein that is no longer able to control cell growth and division The CDC73 gene allows for the making of the parafibromin protein which is a tumor suppressor as well. A mutation here causes a decrease of the parafibromin protein. The CASR gene is involved in the production of the calcium-sensing receptor protein (CaSR).…
I-Kappa-B-alpha which is often abbreviated IkBa is a protein whose role is to mask the nuclear localization signals to inhibit the NF-kB (nuclear factor kappa beta) proteins by keeping them in inactive states and isolating them in the cytoplasm, this inhibits the NF-kB transcription factors and blocks the NF-kB transcription factors from binding with DNA. NF-kB is a protein complex that is responsible for controlling cytokine production, transcription of DNA, cell survival, and plays a key role…
When administered, sugammadex forms a water-soluble complex with aminosteroid non-depolarizing agents, thereby reversing NMB.22 Sugammadex has an affinity for aminosteroids as follows: rucoronium> vecuronium> pancuronium.1 Specifically, sugammadex is 2.5 times more selective for rocuronium than vecuronium.23 Sugammadex acts three to eight times faster than neostigmine to reverse a NMB.24 Due to its highly selective reversal of rocuronium-induced NMB, there is a new but growing interest in…
Background and Education Plans The end, or reason, for my life is something that had intrigued me early in life. As a child, I began to consider the origins of my name and learned that “Alexis” was derived from the ancient Greek word Alexos – which means, “to defend.” Through personal experiences and contemplation, I have made it my firm conviction that the end, or purpose, of my life is to defend and be of service to the people. My career objectives include completing my Bachelor’s degree…
SNARE Proteins Membrane fusion is a function of cell division and cell growth. The machinery used in membrane fusion is also a component of memory and learning. SNARE (soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptors) proteins function in membrane fusion. They also ensure specificity in regulating membrane docking and fusion in exocytosis process and at the pre-synaptic plasma membrane. There are questions regarding how SNARE proteins specificity is enacted and…
co-immunoprecipitation of HEK293T cells stably expressing FLAG-RagB who are starved from 50mins and then re-stimulated with glutamine for 10mins and immunoblot for endogenous FLCN levels. If FLCN is glutamine sensitive, you should see a transition from protein interaction between FLCN and RagB seen under…
wheat kernels in 150 μm sieve; Wwheat in 75μm sieve = weight of wheat kernels in 75 189 μm sieve; Wpan = 190 weight of empty pan; and Wmaize meal = weight of maize flour. 191 Determination of Crude Protein 192 The crude protein content of the roasted maize was determined in duplicate by Dumas combustion 193 method as described in AACCI Approved Method 46–30.01 (AACC, 1999) using a Leco, model 194 FP-528 (Leco Africa, Kempton Park, South Africa). Calibration of the instrument was done using 195…