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    Transferrin is a protein that circulates in the plasma and is encoded by the transferrin (TF) gene. The protein contains 678 amino acid residues, 19 disulfide bonds and has a molecular mass of about 80 kDa. Transferrin has a combination of alpha helices and beta sheets that form an N-terminus and a C-terminus domain (1). The iron ion that binds to the protein binds to two tyrosine, one aspartic acid and one histidine residues. In humans, the major function of transferrin is to regulate the level…

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    Glucose-1 (GLUT-1) deficiency syndrome is an inborn error of metabolism caused by a mutation of the SLC2A1 gene (1). The resulting dysfunctional GLUT-1 protein causes inadequate amounts of glucose to be transported to the brain and results in neurologic disorders such as childhood intractable epilepsy (1). Although introduced nearly a century ago, the ketogenic diet has gained increasing popularity within the last decade to treat epileptic seizures, especially when minimal or no improvement is…

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    Endocrine Tumors

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    1.) According to Jonbarron.org, an endocrine tumor is a mass that begins in the parts of the body that produce and release hormones. The neoplastic cells secrete the hormone normally produces by the cells of the gland, but they are not responding to negative feedback which controls mechanisms so hormones re secreted in an inappropriate and excessive manner. Because an endocrine tumor develops from cells that produce hormones, the tumor itself can produce hormones and cause serious illness. 2.)…

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    Macromolecules Macromolecules are complex carbon based molecules that all have different functions and monomers, there are four different groups of macromolecules that include, Nucleic Acids, Carbohydrates, Proteins, and Lipids. Carbohydrates are polymers made from monosaccharides, which are simple sugars like glucose and fructose, these are used as a good source of energy. Monosaccharides, the monomer of carbohydrates, are just one sugar unit, they are the simplest form of carbohydrates, but…

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    Each lectin molecule usually comprises of two or more carbohydrate-combining sites, i.e., they are di- or polyvalent. Concanavalin A (a tetramer protein originally extracted from the jack-bean, Canavalia ensiformis) is a member of the legume lectin family and is a particular lectin that has been observed to be able to agglutinate a multitude of cell types, including myocytes, B-lymphocytes and fibroblasts, but for it to perform this action, it needs the presences of sugars which include mannose…

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    Insulin Receptor Introduction Insulin receptor (IR) is a transmembrane receptor, an important member to a large family of tyrosine kinase receptor proteins. Endogenous ligands such as insulin, IGF-1 (Insulin-like growth factor-1) and IGF-2 (Insulin-like growth factor-2) bind to this receptor to carry out major physiological and metabolic functions. INSR (Insulin Receptor) gene encodes a preproprotein that is proteolytically processed in our body to form a functional heterotetrameric insulin…

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    The Shakeology shake is hands down the most nutritious and all rounded shake in the market today. At only $4, one serving of this delicious shake is equivalent in nutrition value to the following; Romaine lettuce- 3 cups Raw broccoli- 4 cups Exotic fruit- 1 bowl Raw cauliflower- 10 cups Raw mushrooms- 4 cups Wheat grass- 1 shot Raw onions- 3 pieces Raw carrots- 7 pieces Peas- 1 cup Red radishes- 4 cups Nonfat yoghurt- 4 cups Market research shows that purchasing all these ingredients separately…

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    GBA1 MUTATIONS INTERFERE WITH WNT/β-CATENIN SIGNALING AND LINEAGE DIFFERENTIATION IN GAUCHER IPSC-DERIVED NEURONAL PROGENITORS. Robert Liu*, Ola Awad, and Ricardo Feldman, Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD. Gaucher disease (GD) is an inherited lipid-storage disease caused by mutations in the acid beta-glucocerebrosidase gene (GBA1). Mutant glucocerebrosidase (GCase) leads to the accumulation of glucosylceramide and neurotoxic…

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    12-Well Tissue Culture

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    A 12-well tissue culture plate was used to carry out the LIVE/DEAD Assay. Well “A” contained the untreated HepG2 cells. Well “B” contained camptothecin, which is used as a control for killing the HepG2 cells instead of the use of plumbagin. Well “C” contained the compound Epigallocatechin Gallate. Well “D” remained empty, because a vehicle treatment is not needed for this experiment. Each well contained 1 ml of HepG2 cells. A negative and positive control where both set up in the 12-well tissue…

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    As briefly mentioned before, atropine acts as an antagonist on acetylcholine because its chemical structure is similar to acetylcholine. Thus, structure is an important feature of the atropine molecule that is imperative to understanding its effects on the body. Although today’s chemists know the chemical structure of atropine, the conformational flexibility of the molecule is still poorly understood. A conformation is any three-dimensional arrangement of atoms within a molecule that results…

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