Western blot

Overall, there would be four Western blots completed. There would be two experimental Western blots completed: blot one would contain the ER fraction and blot two would contain the transmembrane fraction. Both blot one and two would be exposed to primary and secondary antibodies (one primary Ab would be specific for MH1 and one primary Ab would be specific for MH2). There would be two control Western blots completed: blot three would contain the ER fraction and blot four would contain the transmembrane fraction. Both blot three and four would not be exposed to primary or secondary antibodies (blocks would be used to supplement the…

between organisms means that there is more variance in the amino acid sequencing. However, proteins do most of the cell’s work and they also function as an immune antibody. The immune system consists of diverse cells and antibody molecules. Antibody is blood proteins that get activated when foreign organisms invade the body. They recognize certain antigens based on their epitope, which is usually five to eight amino acids long on the surface of the protein. Western blot is used to visualize the…

The nitrocellulose piece was first submerged in nanopure water. Then, the blot sandwich was assembled in a tray of transfer buffer. One side of the transfer clamp was placed on the bottom of the tray and filled with transfer buffer. One Scotchbrite pad was submerged onto the bottom of the transfer tray. A piece of blotting paper was submerged onto the pad. The gel was then removed from the cassette and the top left corner was clipped. The gel was laid on top of blotting paper with the clipped…

filling? Antigens 15. Describe some advantages of using the ELISA technique to process a large number of samples. The advantage of Sandwich ELISA is that the sample does not have to be purified before analysis, and the assay can be very sensitive. 16. Which patient(s) were positive for HIV? Patient C is HIV positive 17. Describe what would happen if you skipped the step where the developing buffer was added. The developing buffer contains the secondary…

Objectives Objective 1: Develop a cell line expressing the duck viperin. In order to develop an avian model that is able to express the duck viperin with an advantage of stable and homogeneous expression, duck viperin with and without the C-terminal V5 tag was previously cloned into the mammalian expression vector pcDNA3.1/Hygro+. Then, with the use of lipofectamine 2000, I stably transfected an empty vector and duck viperin tagged or untagged into DF-1 cells. In order to get a monoclonal…

a Western Blot must be done to ensure that the antibodies detected in the ELISA test are really HIV antibodies. The Western Blot test can come back positive, negative, or inconclusive. After you had a seemingly positive ELISA, s/he used the Western Blot to dig a little deeper and get a more reliable answer. The negative result from the Western Blot is definitive. About 0.2 percent of ELISA tests give positive results that are then proven false by the Western Blot test. An ELISA test can be…

This book tells the story of Damon Courtenay as he tries to overcome the adversities caused by haemophilia and Aids. After I finished this book, I was disappointed by the treatment people with HIV and Aids received, particularly by the men and women in the medical profession. I don't want to sound hypocritical and judge these people because personally, I don't know how I will react to such situation, particularly in the 1980’s where the knowledge of this disease was very low. I also believe…

There was another discovery where the DNA sequences of HPV 18 were detected in cell lines derived from cervical cancer. The objectives of the investigation that took place in the article “Structure and transcription of human papillomavirus sequences in cervical carcinoma cells” was to analyze the cell lines HeLa, C4-1, and 756, for the structural organization and transcription of the HPV 18 genome. The methods that were used in this investigation was to first isolate RNA and subject it to…

Western’s a genre with a purpose For almost as long as the medium of filmmaking has existed so too has the Western first showcased in Edwin S. Porter film The Great Train Robbery. Though overall a simple film in retrospect the way in which it pushed the medium forward was revolutionary in containing a narrative. Cowboys are the initial American heroes of filmmaking which all others pull from; Westerns as a male focused genre the central genre trope of masculinity have been constructed on a…

The following essay will compare the cinematic language of the two Western classics Stagecoach (John Ford, 1939) and The Good, the Bad and the Ugly (Sergio Leone, 1966) while analyzing the claim that both film respectably are pioneers of the Western genre during their times proven on the basis of their original work in editing and narrative, and its influence on other filmmakers. After a brief summary of both movies, I will continue with the analysis of both, in particular with the formal…