Western blot

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    Overall, there would be four Western blots completed. There would be two experimental Western blots completed: blot one would contain the ER fraction and blot two would contain the transmembrane fraction. Both blot one and two would be exposed to primary and secondary antibodies (one primary Ab would be specific for MH1 and one primary Ab would be specific for MH2). There would be two control Western blots completed: blot three would contain the ER fraction and blot four would contain the transmembrane fraction. Both blot three and four would not be exposed to primary or secondary antibodies (blocks would be used to supplement the…

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    Western Blots

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    between organisms means that there is more variance in the amino acid sequencing. However, proteins do most of the cell’s work and they also function as an immune antibody. The immune system consists of diverse cells and antibody molecules. Antibody is blood proteins that get activated when foreign organisms invade the body. They recognize certain antigens based on their epitope, which is usually five to eight amino acids long on the surface of the protein. Western blot is used to visualize the…

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    Homogenate Lab Report

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    The nitrocellulose piece was first submerged in nanopure water. Then, the blot sandwich was assembled in a tray of transfer buffer. One side of the transfer clamp was placed on the bottom of the tray and filled with transfer buffer. One Scotchbrite pad was submerged onto the bottom of the transfer tray. A piece of blotting paper was submerged onto the pad. The gel was then removed from the cassette and the top left corner was clipped. The gel was laid on top of blotting paper with the clipped…

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    filling? Antigens 15. Describe some advantages of using the ELISA technique to process a large number of samples. The advantage of Sandwich ELISA is that the sample does not have to be purified before analysis, and the assay can be very sensitive. 16. Which patient(s) were positive for HIV? Patient C is HIV positive 17. Describe what would happen if you skipped the step where the developing buffer was added. The developing buffer contains the secondary…

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    Tpk-Trypsin Lab Report

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    Objectives Objective 1: Develop a cell line expressing the duck viperin. In order to develop an avian model that is able to express the duck viperin with an advantage of stable and homogeneous expression, duck viperin with and without the C-terminal V5 tag was previously cloned into the mammalian expression vector pcDNA3.1/Hygro+. Then, with the use of lipofectamine 2000, I stably transfected an empty vector and duck viperin tagged or untagged into DF-1 cells. In order to get a monoclonal…

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    This book tells the story of Damon Courtenay as he tries to overcome the adversities caused by haemophilia and Aids. After I finished this book, I was disappointed by the treatment people with HIV and Aids received, particularly by the men and women in the medical profession. I don't want to sound hypocritical and judge these people because personally, I don't know how I will react to such situation, particularly in the 1980’s where the knowledge of this disease was very low. I also believe…

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    There was another discovery where the DNA sequences of HPV 18 were detected in cell lines derived from cervical cancer. The objectives of the investigation that took place in the article “Structure and transcription of human papillomavirus sequences in cervical carcinoma cells” was to analyze the cell lines HeLa, C4-1, and 756, for the structural organization and transcription of the HPV 18 genome. The methods that were used in this investigation was to first isolate RNA and subject it to…

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    The Searchers is a “classic” western. It tells the story of Ethan Edwards who is accompanied by his nephew Martin Pawley and their five-year-long search to find his niece after she is captured and her family killed by the Comanche Indians. Little Big Man is a “revisionist” western. It tells the story of Jack Crabbe, a 121-year-old man asked to tell his story about his life, which includes being captured and raised by the Indians and living in a white society with a variety of jobs leading him to…

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    Western Film “For over forty years, from 1926 to 1967, Hollywood produced more Westerns than any other kind of film” (pg.243). The western film I chose was one of Sergio Leone’s famous trilogies, which included A Fistful of Dollars, For A Few Dollars More, and The Good, The Bad, and The Ugly. These are known as Spaghetti Westerns. The film The Good, The Bad, and The Ugly takes place during the American Civil War and follows three men on the hunt for Confederate gold. Many westerns focus…

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    coming from the back or the side of the main characters” (Goodykoontz & Jacobs, 2014, section 6.4). Ultimately, the type of lighting has assessed the impact of high contrast and very deep shadows in many of the indoor/outdoor scenes to establish the western theme. ◦What are the benefits of the style of lighting used? The benefits of the lighting style used to enhance the hardness of life on the Western frontier, therefore, giving the movie viewers a real sense of the western time and its…

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