CHAPTER THREE MATERIAL AND METHODS 1. PHYSICO-CHEMICAL CHARACTERCITICS: The effluent collected were analyzed in the laboratory for pH, acidity, alkalinity, salinity, total dissolved solid(TDS), conductivity, total hardness, calcium hardness, magnesium hardness, total solid, total suspension solid, turbidity, BOD, DO and COD using standard methods (APHA,2005). A.DETERMINATION OF pH: The electrode of pH meter was calibrated with standard buffer solutions of pH 4, 7 and 9. The electrode was then…
To determine optimal pH value for the hydrolysis of p-NPG by leukocyte GCase, we examined effects of variable pH (4.0-5.5) at a constant substrate concentration (5mM). Figure 1 shows the pH activity curves of leukocyte GCase assayed in the presence and absence of 6 mg per ml Sodium taurocholate. In the absence of the pure sodium taurocholate, the activity in the range of pH 4.0-4.8 was considerably lower. However, in the presence of pure Sodium taurocholate, leukocyte GCase was optimally active…
Truc-Nhi Do Separation of a Mixture The purpose of this lab was to separate a mixture, calculate the percent composition by mass, and find percent error. I first started out by taking the mass of each component of the lab. I used the infiltration method to allow the salt and water solution, a blended mixture, to pass through the filtration paper and into the beaker. A trick to know if something is a solution is to see is it has the aqueous sign (aq) in the formula, like NaCl(aq). This step…
Contents: Introduction-Page 2 Review of Literature- Page 3 Presenting of findings- Page 4 Processing of findings- Page 5 Conclusion- Page 8 Reference list- Page 8 Appendix-Page 9 The research that I have conducted on acid mine drainage (to be referred to as AMD throughout the contents) has been to investigate the effects of AMD on the environment with a more definite focus on the effects that AMD has on plant life. The aim of this research is to determine the impact that AMD has on the…
neutralize an acid in a reaction. Antacids are bases and the chemistry definitions of a base vary. A base is defined as a substance that produces OH- ions in solution in the Arrhenius definition. There is also the Bronsted Lowry definition and the Lewis definition. They are defined as the following respectively: a proton acceptor or H+ ion acceptor and an electron pair donor. These terms are used interchangeably to figure out if an element or compound is a base. A base will produce OH- in the…
Summary: Put on any necessary personal safety equipment, and one person from each team should collaborate with people from other teams to create the NaOH solution. The other team members should focus on setting up the buret by rising it with distilled water as well as building the titration apparatus and preparing the HCl solution. In order to prepare the NaOH solution, first take some solid NaOH and measure its mass. Then get a 250 mL volumetric flask and fill it about halfway with distilled…
Abstract The main goal of the buffer capacity experiment was to analyze how the addition of acid or base and dilution affected the measured pH. The results showed that when the buffer systems were under acidic conditions their pH values decreased and increased when exposed to a basic environment. The main goal of the ionization of the amino acid experiment was to titrate an unknown amino acid with a strong base in order to produce a titration curve. The titration curve was used to evaluate the…
1. There is not much known about phage influence on microbial communities in extreme thermal environments. What may be one of the roles these phages have in this environment? - Phages are an important components of surface hot springs, they are abundant and active components of hot springs capable of killing a significant proportion of the resident microbial populations. They play an important role as predators of hot spring microbial communities and influence carbon and nutrient cycling in…
M) * MM(CH3COOH) (0.5967M) * (65.05 g/mol) = 35.85 g/L 4) Percent Acid ((g/L)/(given density)) * 100 ((35.85 g/L)/(1005 g/L)) * 100 = 3.57% UNKNOWN ACID 1) Moles of NaOH ((Vf - Vi)/1000) * M Run #1: ((31.11 – 0.51)/1000) * 0.2116m = .006475 Run #2: ((31.35 – 0.38)/1000) * 0.2116m = .006553 2) Calc Moles of Acid because 1:1 ratio moles acid equals moles NaOH moles acid = Run 1: .006475 and Run #2: .006553 3) Molar Mass (grams used/moles acid) Run #1: (1.3160g/…
“normal” range for the human body? If not, when was the pH value outside of the normal range, and what acid/base imbalance did this pH value indicate? No, acidosis occurred at 40 seconds when the pH dropped out of “normal” range to 7.29. 6. Did the PCO2 level change during the course of this run? If so, how? Yes, the Pco2 level elevated. 7. If you observed an acid/base imbalance during this run, how would you expect the renal system to compensate for this condition? …