Vacuum flask

Stericup Sterile vacuum filter units). Once all filtered processes were complete the samples was covered with parafilm and store in -4degree C freezer. Then nine 2L flask was labeled the following: ECR20,ECR100,ECR200,ECS20,ECS100,ECS200, L20,L100,L200. The ECR represented E. crassipes root, ECS represented E. crassipes shoot, L represented Lemna sp. Then each extract was brought to the volume of 1280 ml. In the sample of 20 ppt 80ml of extract was added to 1920 ml diH2O in a 2 L flask. In…

culture flask with 1.5 ml 100x hippos. The culture flask was filled with DMEM up to 150 ml and this will be the medium without the PSG and this process completed the medium without the PSG. The same procedure was followed for the PSG medium, 1.5 ml of PSG was added and that completed the medium with PSG. Bacterial culture plates for NFkb and GFB 3.5 g of 7 g/250 ml agar and 6.5 g of 25 g/100 ml LB was added to a 500 ml flask. 250 ml of distilled water was added to the same flask. The flask was…

involves several steps, selecting an ideal solvent, dissolving the compound in a minimum amount of boiling hot solvent, filtering insoluble impurities while the solution is hot; crystallizing the purified compound, and isolate the pure compound by vacuum filtration . The unknown used in the experiment was #4, which were white crystals. In order to determine the ideal solvent we prepared two test tubes. Test tube #1 containing 0.106g of unknown and 3mL of water, it was well mixed. At room…

A small burette funnel was filled with column packing solvent at mobile phase, high boiling petroleum, in this case hexane (and then 10%acetone hexane mixture), and Alumina as stationary phase. When adding the sand and the alumina they have to be first mixed with the hexanes in order not to disturb the column. The Lycopene is Red- Orange and more attracted to alumina due to its high degree of Unsaturation, which can be expected to be eluted after Yellow-Orange Carotene pigments. And the other…

Then 0.12g of benzoyl peroxide was added. The flask was swirled until it was a homogenous mixture. Next, the reflux apparatus was set up. After set up was completed, the round bottom flask was heated in a heating mantle attached to a transformer that was set to 80%. The reaction was heated until the reflux began. The reflux was maintained for 30 mins without the condensation…

into a round-bottomed flask. The round-bottom flask was then moved to the hood where the it was attached to a distillation column, placed on top of a sand bath. The sand bath temperature was placed between 30C to 40C. At the initial sight of reflux, a timer was set for 40 minutes. The mixture’s appearance was initially green, but darken over time as the experiment proceeded. After 40 minutes, the distillation column was disconnected from the round-bottomed flask. The flask was left alone for 10…

This experiment was conducted to practice a variety of techniques in organic chemistry: separation, mechanisms, acid-base reactions, evaporation, recrystallization, and identification. The goal of this experiment was to identify the unknown mixture of two compounds. The two compounds were separated first by altering the acid components solubility through deprotonation, and then protonating the acid component again to form the separated precipitate. The ether in the neutral layer was evaporated,…

The flask was attached to a reflux condenser and the mixture was reflux for 45 minutes, during which butadiene sulfone was decomposed. The mixture was allowed to cool and then transfer to another 100 mL beak, with the addition of 10 mL of tetrahydrofuran. A small amount of charcoal was added to the solution, mixed, and vacuum filter. Hexanes (50 mL) were added to the filtrate, cooled in an ice bath, and vacuum filtrate once more. The precipitate was washed…

injection of the precursors solution in a three-neck flask containing alcohol. Briefly, the alcohol solution containing ODA (25.45 g) and ODE (150 mL) was load in a 250 ml three-necked flask, degassed at 120 °C for 2 h, followed by heating at 310 °C. Then, a typical solution precursor containing 0.86 g of InAc and 5.2 g OA in 12.5 mL of ODE was prepared separately by degassing and heating to 120 °C. At this temperature, the solution was evacuated using a vacuum pump and refilled with Ar gas to…

distillation used for temperature sensitive materials like natural aromatic compounds which are insoluble in water, but volatile in steam. The non-volatile impurities are left behind in the flask. It once was a popular laboratory method for purification of organic compounds, but has become obsolete by vacuum distillation. Figure 6 Steam Distillation EXTRACTION WITH A SOLVENT The process of removing or separating a substance from its aqueous solution using a suitable organic solvent is called…