Transmembrane protein

Phase 2 – Agarose Gel Electrophoresis 1. Obtain our four H, E, L, and P labeled micro tubes and place them on ice. 2. Set the micro pipet to 2.0 ul. 3. Transfer 2.0 ul blue dye to each of the four H, E, L, and P micro tubes. 4. Mix the four micro tubes by using this two-step method: * Place each tube briefly on the vortex to get the components to collect at the bottom of the micro tubes * Pulse spin the four micro tubes in the centrifuge to mix all of the components completely. 5.…

4.1 Maintenance and sub-culturing of endophytic fungi The cultures were procured from already maintained repository in laboratory. The maintenance of the cultures involved preparation of Potato dextrose agar (PDA) plates, sub culturing of cultures and long term preservation. 4.1.1 Preparation of PDA (Potato dextrose agar) plates 39g of PDA was dissolved in 1000 ml double distilled water, stirred to mix properly and was transferred into 250 ml Erlenmeyer flasks and autoclaved at 121˚C at 15…

1.4 Effect of Microcrystalline Cellulose (MCC)Type Formula F12 was utilized to study the effect of changing the type of MCC(Avecil PH-101 ) as a carrier material instead of (Avecil PH-102) in F8 on the dissolution behavior of the prepared Zolmitriptan liquisolid ODTs. Although the DT of formula F12 (79 sec.) was greater than F8 (48 sec.) as in Table(18),the dissolution behavior of the formula F12 was faster than F8 as in Table (18) and Figure (48), this difference was non…

Salts and molecules have similar characteristics, and many differences whether we can tell by first looks or not. Testing these two only supports the differences, and many structural functions cause these differences. Since salts are ionically bonded, they have different properties and functions compared to the covalent bonded molecules. Salts have a ionic bond, and we can confirm due to their electronegativity. As you move from left to right on the periodic table you would notice that…

Since it is large you can imagijne that it will have a lot of functions such as, it stores vital energy and nutrients, it manufactures proteins and also manufacvtures enzymes that is necessary for good health, it protects the body from diseases, it also breaks down or metabolizes and it removes harmful toxins, such as alcohol, from the body. Since the liver is the major organ for removeing…

Discussion The glycemic index of the dosa prepared from foxtail millet was low. This is due to the high levels of soluble dietary fiber in the millet. The total dietary fiber was reported to be27.02%, soluble dietary fiber 11.56% and the insoluble dietary fiber 15.44%11. It has been reported that the high viscosity of the soluble fiber delays digestion and absorption18. The glucose levels can be maintained to near normal with the help of dietary modification. It can be inferred from the above…

Soxhlet Fat Analysis Introduction The aim of carrying out the soxhlet practical was to find a percentage for the amount of fat in a small portion of minced meat. Soxhlet extraction is one of the most commonly used methods for determining the amount of fat in food products. The method for the procedure is fairly straight forward and most commonly used for the fat content to be recognised. The soxhlet extractor was invented in 1879, by Franz Van Soxhlet. The experiment was typically designed…

ABSTRACT The reactivity of acid base cements forming hydroxyapatite (HA) such as, β-tricalcium phosphate (β-TCP) and dicalcium phosphate dehydrate (DCPD). Amorphous calcium phosphates, prepared by precipitation from supersaturated solutions, can also react to form apatitic cements since they are thermodynamic unstable with respect to HA and have a setting reaction more independent of particle size. Calcium phosphate cement containing an antibiotic can be used for filling bone defects and to…

3.3 Results and Discussion 3.3.1 Agarose Particles By microscopy observation, both ABT and CSS were activated by the Mix&GoTM solution with significant colour change on the particle surface. Seen from Figure 3-1 and 3-2, a dark green layer was formed outside the particle after treating with Mix&GoTM. By visual observation, the agarose particles turned from colourless to green, which supported the microscopy observation. The activated particles were also sent for zeta potential measurement and…

The human genome is composed of millions of molecules of DNA perfectly packaged into 23 chromosomes. Each human is the result of a combination of the same four nucleotides, yet every single one is unique. Perhaps, this is due to the slight variation in each human’s DNA, or maybe the explanation lies in a person’s upbringing. The purpose of studying human development is to understand why people change by applying the scientific method to existing theories, which provide the basis for…