Transmembrane protein

Proteins Introduction Many products including powders, drinks, and foods are marketed as having more protein in them than the average consumption. But is that statement actually true? Proteins are a type of amino acid. There are twenty known amino acids monomers. Monomers are molecules that can be bonded together to create something else known as a polymer. Every amino acid has to have three things, an amino group, a carboxyl group and a functional group that is special to itself. Something…

Biochemical foundation for each of the four enzymatic assays performed on each cellular fraction. We performed various assays on each of the four log phase culture fractions, as well as the extracellular fraction from a stationary phase culture, to test the localization of the enzymes. For the protease assay, a specific soluble chromogenic substrate called azocasein was employed to determine presence of enzyme activity. The degradation of this substrate in trichloroacetic acid produces a colour…

DNA codes for protein by protein synthesis. In protein synthesis, there are two major steps; transcription that creates mRNA and translation that translation mRNA to translate into protein. Transcription happens in the nucleus, after the process of transcription, mRNA molecule is then sent to the ribosome where the message is translated into specific proteins. This process is called gene expression. Millions of cells in the human body…

Q1) What secondary structures make up the large C-terminal domain of the Klenow fragment? Answer: The large C-terminal domain contains a six-stranded antiparallel β-sheet at the bottom of the cleft region and contains three α-helices on one side and four α-helices on the other side of the cleft region. Q2) Describe the three-dimensional structure of the large C-terminal domain of the Klenow fragment? Answer: The large C-terminal domain contains a significantly deep cleft, with a considerable…

fourteen different organisms. The data produced was an identification table, a sequence alignment diagram and a cladogram. In cladograms the branches are not representative of the evolutionary time that has occurred. Heat shock proteins (HSPs) are in a class of proteins called molecular chaperones. (Kiang, J.; G, Tsokos. 1998). They are present in both eukaryotes and prokaryotes, in the cytosol, mitochondria, endoplasmic reticulum and the nucleus. In humans they have a relatively long…

vegetarian diet is one lacking red meat, poultry and seafood. Whether or not vegetarianism is good for one’s health tends to be a controversial topic, as meat contains more essential nutrients than vegetables. Meat provides a higher-quality source of protein than vegetables because meat is packed with amino acids that vegetables do not supply. The type of iron in meat also absorbs better than vegetables which contributes to low iron levels in vegetarians. Due to these facts many believe that…

FPV is in the family Parvoviridae, which contains 26 species of virus that infect a broad host range, covering species from invertebrates to humans. Parvoviruses are small single stranded DNA viruses enveloped by a T=1 non-enveloped capsid protein. Replication of these viruses can only occur when the host cell is in S-phase of its mitotic cycle (15). FPV has a very small linear genome that is only 5 kilobases in length (4). The virus’s genome has specific sequences, similar to a TATA boxes, that…

Sarah Diaz Dr. Basu Bio 572 My Favorite gene One of my favorite genes that I have had the pleasure of working with is known as PROMININ-1, or PROM-1. One of the reasons why it exists as my favorite gene, is because the research lab that I am interning at studies this gene and its role in a very well known and unfortunate disease, biliary atresia. It also is one my favorites due to the fact that it has a lot of potential applications in studying other diseases or further research. These…

Specific activity is useful to know for a protein as it is a good indicator of an enzyme 's purity and quality. The Bradford assay measures total protein in the assay, and was calculated as 4.2mg/mL. The result was calculated from a set of four concentrations, of which there was a standard deviation of 1.3mg/mL and a standard error of 0.66mg/mL. These…

species owe their success to various forms of anti-freeze proteins. Within Northern Atlantic resident species, there are six classes of Anti-Freeze Proteins (AFPs), generally categorized by molecular size, structure and effectiveness. AFPs are a remarkable exhibition of convergent evolution – there are vast multitudes of AFPs, all present in species with no significant common ancestry. Additionally, there is no ‘common ancestry’ in the proteins themselves; some are fibrous, some are globular,…