Transmembrane protein

leukemia 1 protein (AML1) or core-binding factor subunit alpha-2 (CBFA2) (10, 11). It is one of three RUNX proteins found in humans, all of which possess a highly conserved 128 amino acid Runt DNA-binding domain at the N-terminus. The Runt domain is important for binding with consensus sequence (PyGPyGGTPy) in DNA and with core binding factor subunit beta (CBF-β) (12). CBF-β heterodimerizes (13) with RUNX1, enhances RUNX1 affinity for the DNA by 10 fold (14) and stabilizes RUNX proteins against…

Introduction According to Bio-Rad, Alamar Blue is a “cell viability assay reagent which contains the cell permeable, non-toxic and weakly fluorescent blue indicator dye called resazurin.” (Bio-Rad, n.d.). Meaning that the resazurin is going to be using an oxidation-reduction (REDOX) indicator that will undergo a color change due to the response of the cellular metabolic reduction. “The reduced form resorufin is pink and highly fluorescent, and the intensity of fluorescence produced is…

Enzymes are protein molecules that significantly speed up the rate of virtually all of the chemical reactions that take place within the cell. In the following lab was to examine an enzyme that is found in pineapples. That enzyme is bromelain its breaks down protein into their protein into their amino acid by a process of hydrolysis. They are found in tropical areas like Hawaii, Thailand, and Brazil. For the first experiment the hypothesis was that the when gelatin was added to the water, fresh…

removed and a covalent bond is formed. Breaking this bond is called hydrolysis, it is the the addition of a water molecule and releases energy. Examples of macromolecules would be nucleic acids, proteins, and polysaccharides (2 or more sugars). The biological components are composed of starches, proteins, lipids, and nucleic acids. Starches consisit entirely of glucose monomers and are joined by glycosidic linkages, only found in plants. Lipids are a large group of biological molecules that…

Not to mention, the safety of whey protein supplements largely depends on the dose; even with that coming into play, most Americans eat or drink twice as much protein as they need, and any extra protein will be stored in the body as fat and can cause an increased risk of dehydration. Too much protein can also lead to a person’s calcium loss or osteoporosis. When this happens, acids are released into the bloodstream when the protein is digested, and the calcium is used to eat those acids,…

The Effect of Dri-Contrad on Phagocytosis in Tetrahymena Methods: A control group of Tetrahymena was first made up of 450 microliters of Tetrahymena and 450 microliters of carbon solution in a microcentrifuge tube and a timer was immediately started. 100 microliters of iodine were added to three other microcentrifuge tubes which were labeled C3, C6, and C9 to act as a stop solution by killing the Tetrahymena. At three minutes, 100 microliters of solution were pipetted from the control tube to…

Separation of plasma proteins by molecular exclusion chromatography Sebastian Prisacariu 140013826 Introduction The components of blood is broken down into 3 parts. This breakdown is consisted of: Plasma which is a yellowish fluid that helps transport blood cells around the body and contains a small supply off proteins, platelets which are blood clotting agent, red blood cells which is a carry for oxygen and remover off carbon dioxide and white cells which is the bodies defence from infection…

In sequence 2, the DNA coded for the gene Apolipoprotein E, also known as APOE. The APOE protein is made from instructions from the APOE gene. This protein binds with fats from the body to form lipoproteins, which are responsible for packaging cholesterol and other fats and carrying them through the bloodstream. There are three different versions of the APOE gene. They are known as APOE e2, e3, or e4. Different forms of APOE have been shown to cause a variety of different diseases. The most…

Lipoproteins consists of cholesterols, lipids, phospholipids, and proteins. When one talks about "good cholesterol" verses "bad cholesterol", what one is actually talking about are one of two lipoprotein chains. A lipoprotein chain that has a high amount of proteins as compared to a lower amount of lipids is called a High Density Lipoprotein (HDL). Lipoprotein chains that have a higher amount of lipids as compared to a lower amount of proteins is called a Low Density Lipoprotein…

1. Flavin monooxygenase (FMO) and cytochrome P450 (CYP450) are both enzymes that are involved in phase I metabolism. They are classified as microsomal enzymes that require oxygen and NADPH. These enzymes are involved in the conversion of lipophilic compounds to more hydrophilic metabolites by adding molecular oxygen, which ensures rapid excretion. There are genetic variants with both classes of enzymes, which may contribute to interindividual variability in drug response. Some of the…