Transmembrane protein

Purpose: The overall goal of this lab was to perform a procedure on E. Coli which involved transferring genes that encoded for the green fluorescent protein into E. Coli to see if the transferred genes would make a difference on the growth and whether or not the bacteria would glow under UV light. Hypothesis: If the bacteria with the pGLO plasmid was grown on a plate containing LB and ampicillin then the bacteria will grow but not glow under UV light. If the bacteria with the pGLO plasmid was…

2.8.4 Properties The application of carrageenan as food additive depends on its performance such as forming thick solution or gels. For example, κ− carrageenan forms strong and rigid gels with potassium salts, forms brittle gels with calcium salts, clears gel with sugar addition, and exhibits syneresis. On the other hand, ι− carrageenan forms elastic gels with calcium salts, clears gel with no syneresis, and creates a gel that is freeze/thaw stable, while λ− carrageenan is not gel-forming but…

Direct Coombs test is also called Direct Antiglobulin Test or DAT. This test is used for the detection of IgG or C3 antibodies which are attached to the RBC’s surface. These antibodies are important to be detected because they might sometimes cause anemia due to destruction of RBCs. DAT is mainly done on blood samples of newborns, especially when a newborn is suffering from jaundice. • Procedure: 1. Place the sample of RBCs to be tested in a 5 % suspension of isotonic saline. 2. Using a…

extraction the proteins absorb light at 260nm and 280nm, especially the aromatic acids. 2. 250µg to nm, 250 x 1000 = 250,000ng. 3. 200ng/ml = 0.2µg/ml. 150µg/ml = 150,000µg/ml 250,000pg/µl = 250µg/ml 1mg/ml = 1000µg/ml ● 150,00µg/ml - HIGHEST CONCENTRATION OF DNA ● 1000µg/ml ● 250µg/ml ● 0.2µg/ml - LOWEST CONCENTRATION OF DNA 4. Sodium dodecyl sulphate is used as a detergent, which allows the cells to release DNA by disrupting the cell and nuclear membranes. It dissolves the…

Ethanol disrupts membranes by a number of methods. In particular what you must remember is, the size and fluidity of the membrane has a lot to do with the outside and inside environment. This environment consists of how much water, salts etc are in the cell versus out of the cell. Adding ethanol disrupts much of this. 1. Ethanol is permeable to cell membranes. Because of the nature of ethanol as a chemical is it able to get into the cell very easily. This disrupts the balance of the…

Deoxyribonucleic acid consists of a phosphate and deoxyribose backbone, and four different nitrogenous bases. These bases are adenine, guanine, cytosine, and thymine. Ribonucleic acid, the molecule formed in the transcription of DNA, is made up of phosphate, ribose, and adenine, guanine, cytosine, and uracil. The backbone of DNA consists of a phosphate bonded to a deoxyribose molecule. Deoxyribose is a sugar containing five carbons, and it is called deoxyribose as it is missing an oxygen atom on…

The Effect of Lime Juice on a Gummy Bear’s Mass Lab Introduction Objective: In this lab we are going to learn the effect of lime juice on a gummy bear when it is soaked for 24 hours. We will see the effect is has on the mass when it is taken out. Information is Useful: This lab is an example of how cell transport works. The gummy bear is a cell in this case which is placed in a hypotonic solution that is the lime juice. Hyp0tonic solution is a greater concentration of the solute molecules…

because it provides a stable, protective covering to protect the genome from any chemical or physical agents from harming it. In addition, the icosahedral shape is made up of identical subunits, the virus will better utilize its genome to encode proteins for the capsid. Finally, the last reason that I believe icosahedral structures have been favored by evolution is simply because they allow delivery of the genome by specific binding to external receptors on the host cell, which transmit specific…

result for polysaccharides. After that, I preformed the Biuret test, which sees if a substance contains proteins. I first placed 5 drops of each stomach content into the appropriately labeled tube. Next, I added one drop of Biuret solution into each tube. Then, I recorded the result, if the substance changed purple then it contained proteins. Only stomach two and three had a positive trace of protein. With these results I was able to conclude that stomach content one belongs to Margaret…

macromolecule is a molecule that contains any "large number" of atoms. There are four main different categories of macromolecules that make up our body: carbohydrates, lipids, proteins, and nucleic acids. (1) Carbohydrates are sugars that store and transport energy. (2) Lipids also store energy and also are signalling molecules. (3) Proteins are linear chains of amino acids that help with the structure, function, and regulation of the body’s tissues and organs. (4) Finally, Nucleotides make up…