Transcription

The purpose of this lab report is to discuss experiments performed in order to study the ctrA gene. It was hypothesized that ctrA will be present in the four bacterial species studied, which are Rhodobacter sphaeroides 2.4.1, Rhodobacter sphaeroides 17029, Escherichia coli DH5훂, and Rhodopseudomonas palustris BisB5. It was also hypothesized that ctrA is more conserved in closely related species and less conserved in not closely related species. The last hypothesis for this study is that the…

through gene expression, would create a polypeptide. DNA decided, that in order to insure the completion of gene expression, she would create messenger RNA that would be called mRNA for short. The process of created this new mRNA was called transcription and would use the information DNA possessed. First, DNA had to decide which gene to transcribe, and because it was her first time transcribing RNA, she decided to transcribe a short gene. The coding portion of the gene was…

Together these three are accomplished transcription in the nucleus of eukaryotic cells. Polymerase I showed no similarities in the coding region. Polymerase II have the most complex of the three type of polymerase, reflection is a huge diversity of genes and are transcribed by the polymerase. The…

Prokaryotes can make one big mRNA that will be translated into multiple proteins at once, whereas eukaryotes make an mRNA per gene. A repressible operon is when transcription of an operon, like trp operon, is usually on but can be turned off when a small molecule binds to a regulatory protein. On the other hand, an inducible operon is when the transcription is usually off, but can be turned on when a certain molecule binds to a regulatory protein. A good example would be lactose. When lactose is…

cytosines leads to the condensation of chromatin meaning that it may cause the chromatin to be more tight and packed so it will promote gene silencing since it will not allow the chromatin to be expressed; however acetylated histones will promote transcription and thus reversing the effect of the methylated cytosine, making this choice incorrect. C. Methylated cytosines and deacetylated histones associated with relaxed chromatin and gene expression This choice is incorrect. Once again, the…

been made in uncovering critical effects of the transcription factors Phox2a, Phox2b, Hand2 and Gata3 on noradrenergic neurons in the past decade. It is now well known that during the embryonic period, these transcription factors coordinately control the specification and differentiation of noradrenergic neurons. These transcription factors have a potential regulatory role on noradrenergic neurons in the adult brain. For example, all four transcription factors are always present in fully…

holds the DNA code. ATG-ACT-CAT-GAG-TGA need to go through the process of the polymerase RNA allowing the transcription to occur. In order for this to occur there has to be a TATA box that tells the TATA to repeat so in order to ATG-ACT-CAT-GAG-TGA there has to be a ATG box that tells the ATG to repeat and then polymerase of the RNA allowing the transcription to occur. In order to have a transcription there has to be a factor. For example, ATG is the first to bind to the ATG box and then binding…

Comparison chart Replication Transcription Purpose The purpose of replication is to conserve the entire genome for next generation. The purpose of transcription is to make RNA copies of individual genes that the cell can use in the biochemistry. Definition DNA replication is the replication of a strand of DNA into two daughter strands, each daughter strand contains half of the original DNA double helix. Uses the genes as templates to produce several functional forms of RNA Products One strand…

Proposed research: Aims and Objectives: Aim1: To address the roles of the Hira complex in rRNA transcription and embryonic development Aim2: To characterise rDNA chromatin organisation in the oocyte Aim3: To elucidate the transcriptional machinery of RNA Polymerase I in the oocyte Experimental Approach: Aim1: To address the roles of the Hira complex in rRNA transcription and embryonic development The Hira histone chaperone complex is an evolutionarily conserved complex, which is composed of…

stage. Multiple transcription factors regulate temporal identity transitions in the SC lineage, but how these factors act in a coordinated fashion remains poorly understood. To better understand cell fate decisions during SC genesis, we compared the temporal expression profiles of a panel of twelve transcription factors from embryonic day (E) 9.0, when neural crest cells (NCCs) are migrating, until E18.5, when pro-myelinating SCs populate the maturing spinal nerve. Two transcription factors,…