Km and Vmax determination of glucocerebrosidase towards p-NPG The Michaelis constant (Km) is a measure of the affinity of the enzyme towards the substrate, with smaller values representing greater affinity. Km and the maximum rate (Vmax) of leukocyte GCase were obtained through the Lineweaver-Burk plot (Figure 4) with artificial substrate p-NPG in concentrations from 0.71 mM to2.50 mM. Km and Vmax values for leukocyte GCase using p-NPG as substrate was found to be 12.6 mM and 333 U/mg…
To determine optimal pH value for the hydrolysis of p-NPG by leukocyte GCase, we examined effects of variable pH (4.0-5.5) at a constant substrate concentration (5mM). Figure 1 shows the pH activity curves of leukocyte GCase assayed in the presence and absence of 6 mg per ml Sodium taurocholate. In the absence of the pure sodium taurocholate, the activity in the range of pH 4.0-4.8 was considerably lower. However, in the presence of pure Sodium taurocholate, leukocyte GCase was optimally active…
The choice of Sydney as the site for the 2000 Olympic Games gave Australia a unique opportunity to reinforce its international standing as a leading sporting nation, promote its image as a free and cosmopolitan society, and develop new trade, business and tourism links with the world. The Sydney 2000 Olymic Games and Paralympic Games were planned and run through a cooperative arrangement between federal, state and local governments. Australian business, and sporting communities, and featured a…
Catherine has developed the project charter and scope statement for this project. . As part of the project charter, Catherine has fully identified the purpose and objectives of the project. She has worked with Fiction Corporation as to the agreed upon scope. Catherine has developed the project deliverables in a scope statement, identified and met with project customers and stakeholders, identified key roles and responsibilities, set up the organizational structure of the project, and identified…
to computer in a loop. C) signals are broadcast in both directions to the entire network. D) signals are broadcast to the next station. Answer: C 35. All network components connect to a single hub in a ________ topology. A) domain B) peer-to-peer C) star D) bus Answer: C 81. Describe the five major categories of IT infrastructure and provide an example of each. Answer: The major components of…
The second mode, the user can have a variety of ways. Recognizing the easiest way is by the couple, each corresponding to two users, the other as a partner. Topology does not correspond to other types of constraints can also be used. This indicates the program has the advantage of simplicity and adaptability. (Anghel and M. Kaveh, 2004, pp. 1416-1421) According to D. Tse and P. Viswanath (2005), signal can…
AN OVERVIEW OF TELEMATICS 1.1 INTRODUCTION Telematics established in 1987 and later acquired by Bahri & Mazroei is a provider of Converged Building Systems, which covers ICT Systems (Information and Communication Technology) like Structured Cabling, Data Networks, Telephoning, etc. and ELV (Extra Low Voltage) Systems like Security, Fire, BMS, Guest Room Management Systems and Car Park etc. Telematics has “ISO 9001:2008; ISO 14001:2004 and OHSAS 18001:2007 certification” and it’s arena…
NIST Special Publication 800-37 Revision 1 Guide for Applying the Risk Management Framework to Federal Information Systems A Security Life Cycle Approach JOINT TASK FORCE TRANSFORMATION INITIATIVE INFORMATION SECURITY Computer Security Division Information Technology Laboratory National Institute of Standards and Technology Gaithersburg, MD 20899-8930 February 2010 U.S. Department of Commerce Gary Locke, Secretary National Institute of Standards and Technology Patrick…