Thin Lizzy

To identify the unknown chemical mixture (#757), a TLC analysis was conducted using three standards (aspirin, acetaminophen, and caffeine). A TLC plate was prepared by drawing an origin line and solvent front line with a pencil. Three spot were labeled A, B, and C. A sample of each standard was spotted on the analytical TLC plate approximately 1cm from the bottom of the plate. It was then enclosed in a jar filled with 2ml of 95% ethyl acetate- 5% acetic acid. (Another laboratory group conducted…

Introduction The purpose of experiment was to identify an unknown analgesic drug using thin-layer chromatography (TLC). Using the thin-layer chromatography to determine the composition of the Acetaminophen, Aspirin, Caffeine and Salicylamide, then compared with unknown analgesics. Experiment Scheme Acetaminophen Aspirin Caffeine Salicylamide C8H9NO C9H8O4 C8H10N C7H7NO Figure 1. The Chemical structure of Acetaminophen, Aspirin, Caffeine…

Investigation 13 “Who Wrote the Ransom Note?” Did The Sticky Bandit Make an Inky Mistake? Introduction: Early Wednesday morning at approximately 6:19 a.m. the widowed Mrs. Regina Butterworth realized when she went to make some syrup that her infamous syrup recipe had been stolen from her secret cookie jar vault. Mrs. Butterworth immediately called the police and upon their arrival they discovered a ransom note left by the alleged sticky bandit. The note was sent to the Tempe CSI Crime Lab…

The purpose of this experiment was to identify the generic name of the unknown drug through thin-layer chromatography analysis. By comparing the Rf values obtained from each TLC plate, it was deduced which components were present in the unknown drug. Through comparison of Table 1 and Table 2, Unknown A was identified as B.C. Tablets. Salicylamide was evident in the unknown substance due to the distinct blue color that was present in both the unknown spot and the salicylamide spot; the similar Rf…

The variable located at the left side of the diagram, the effect of malunggay and ginger root extracts is the independent variable which in being manipulated for the dependent variable, the time spent in treating the sample infected by trichophyton rubrum. The main purpose of this study is to produce an organic antibacterial treatment for Trichophyton rubrum using Malunggay (Moringa oleifera) and Ginger (Zingiber officinale). Specifically, this study aims to answer the following: 1. In which…

Beta-Carotene from Plant Leaves Yokoyama Yuri B1 (A0142349E) 1. Aim: a) To separate beta-carotene and chlorophyll via column chromatography. b) To analyse the contents of the plant extract as well as the separated beta-carotene and chlorophyll via thin layer chromatography (TLC) c) To analyse the absorption peaks of both beta-carotene and chlorophyll using UV-Vis spectroscopy 2. Results: Fig 1. UV-Vis spectroscopy results for S2 (Beta-Carotene extract) Wavelength at peak/ nm Peak obtained/ A…

Isolation of Component Via Extraction Objective: Separation of organic acid from a neutral compound using liquid/liquid extraction. Experimental: Similar to the “Thin Layer Chromatography” experiment, a TLC plate was ran with 2 ml of EtOAc and 8 mL of hexane in the jar. The Rf value was calculated using the formula located in the data for all the TLC plate. The mixed compound that was dotted on the TLC plate had 0.300 g of the impure benzoic acid and 10 mL of dichloromethane. A 50 mL flask…

Abstract The primary goal for this laboratory experiment was to synthesize, purify, and identify p-nitroacetanilide using acetanilide as the starting material. To test for the purity and identification of our desired product, we gathered data from: Thin Layer Chromatography (TLC), Melting Point (MP) range, Infrared (IR) spectrum, Gas Chromatography Mass Spectroscopy (GC-MS), and Proton Nuclear Magnetic Resonance (1H NMR). TLC plate indicated the formation of our pure product due to the…

Methods We began this experiment by laying out the needed materials to find the chromatography of Skittles Sweets and Sours and Crayola markers. Using a ruler and a pencil, a line was drawn on all thirty chromatography strips 1.5 centimeters from the bottom of the paper. This was to ensure that the starting point for each chromatography process was consistent. The chromatography of the skittles was found first. We began by pouring water into a cup. A pipette was used to transfer fifteen…

phase and the stationary phase have similarities or differences that affect the overall interaction between each other and the analyte. Separation of the individual components is achieved because each component travels at different rates. Likewise, Thin Layer Chromatography (TLC) is a chromatography technique where separation of the components in a mixture occurs on a sheet of glass,…