Thin layer chromatography

Aim 1: Determine the amino acid sensing specificity of folliculin. Since folliculin’s has novel molecular function in amino acid sensing I plan to further understand whether FLCN specifically senses a certain amino acid and if this level of detection is enough to elicit an mTORC1 response. 1.1. Determine if folliculin’s novel molecular function in amino acid sensing is triggered by glutamine. mTORC1 activation by amino acids requires FLCN, whereby its lysosomal residence during starvation…

condition. The Pabon method indeed is an easy experiment to perform however, this synthetic procedure yields low to moderate of product. The synthesis of curcumin derivatives that is described by Xu9 has resulted a high purity and yield using Thin-Layer Chromatography (TLC) and ZF-1 ultraviolet analyzer. 2. Medical and Pharmacological…

CHAPTER 4 RESULTS AND DISCUSSION 4.1 Extraction using Different Solvents The grounded Ixora coccinea and Beta vulgaris plant samples were extracted using the following solvents: Acetone, ethanol, methanol and ethyl acetate. The absorbance was noted to select the best choice of solvent for extraction. Inference Acetone, ethanol and methanol showed higher values. But, the dye extracted from acetone and ethanol did not fix to the fabric properly. So, methanol was selected as solvent for…

ether, ethanol, and water). The preliminary phytochemical outcomes revealed that flavonoids and other glycosides as active constituents in ethanol extract of Jatropha gossypifolia. The ethanol extract of Jatropha gossypifolia was undertaken column chromatography with dissimilar solvent fractions. Two compounds were isolated from ethanol extract of Jatropha gossypifolia…

Abstract 6- bromo-2-(4-chlorophenyl)quinazolin-4(3H)-one derivatives 2-7 were synthesized.. The synthesized compounds were evaluated for their anti-microbial activity using the agar diffusion technique . The tested compounds were evaluated against, Gram +ve bacteria , Gram -ve bacteria, Yeast and Filamentous Fungus. Minimum inhibitory concentration (MIC) was made in vitro for compounds which showed antimicrobial activity. Compound 3b has broad spectrum activity against Gram +ve bacteria, Gram…

After the mass of the crude product was measured, the recrystallization was done for more purification; the solution looked clear prior to it’s cooling to room temperature. After the solution cooled to the room temperature and vacuum filtration was done again on recrystallization product, the solution was a white shiny with crystalline solid appearance. The melting point of the Meta-Methyl nitrobenzoate product formed was at 76.2 ‐ 78°C. The appearances of the TLC for the crude product, starting…

Complexation of Cyclodextrins Cyclodextrins (CDs) are most important carriers because of their semi-natural property as it is produced from a natural material i.e. starch, by simple enzymatic conversion. Solubility and dissolution rate of BCS class II drugs can be improved by addition or by complexation with hydrophilic carriers like cyclodextrins. Cyclodextrins have the capability to form inclusion complexes with the guest molecules i.e. poorly water soluble drugs. The formed inclusion complex…

patient takes a plastic cup and urinates in it then then their name is labeled on it. The container is then sent to a laboratory where tests are run. There are several types of urine tests including Enzyme Multiplied Immunoassay Technique, Thin Layer Chromatography, and Radioimmunoassay. The next type of drug testing would be blood testing. The blood tests are performed by a nurse or doctor they stick a needle into the vein of the patient's arm and take a vial of blood. The blood is sent to a…

conditions to its dissociation upon glutamine stimulation. Finally, I will observe FLCN-FNIP RagC/D GAP activity, whereby FLCN complex stimulates GTP hydrolysis on RagC/D when supplied with amino acids. FLCN-FNIP GAP activity will be tracked by thin-layer chromatography (TLC) GTP hydrolysis in protein mixtures of purified FLAG-RagC/D and FLCN-FNIP2 from cell lysates that were either starved or starved then re-stimulated with glutamine, which are then mixed with radio labeled GTP, [-32P]GTP. I…

Interpretation of the NOESY: (Nuclear Overhauser Effect) spectrum of 1-(2-methyl-1,2,3,4-tetrahydroquinolin-4-yl)azepan-2-one (3a) is as follows. Strong coupling interaction of protons of 2nd and 4th position in 1-(2-methyl-1,2,3,4-tetrahydroquinolin-4-yl)azepan-2-one (3a), was not so strongly observed in COSY because it involves long range coupling i.e. protons attached between 2nd and 4th carbon atom. Hence, we studied NOESY to know the spacial interaction between these two protons. Thus, the…