Thin layer chromatography

The heterocyclic compounds naming tetrahydrocarbazoles having the significant biological properties. The newly substituted tetrahydrocarbazole derivatives were prepared from substituted phenylhydrazine and cyclohexanone in glacial acetic acid under reflux. These intermediates on reaction with substituted aromatic acid chlorides in alkaline media finally converted to N-Substituted tetrahydrocarbazoles. Fifteen compounds are synthesized and charaterized by their melting point (MP), IR, NMR, MS…

such as dry leaf, fresh barks of plant, induced callus, somatic embryo and suspension culture (In exogenous medium and in pallet) in 100 mg/ml were analyzed for CPT content by TLC (Thin Layer Chromatography) and HPLC (High Performance Liquid Chromatography). TLC and HPLC analysis confirm the presence of CPT in dry leaf, fresh barks of the plant, induced callus and somatic embryo. Out of which bark extracts possessed a maximum…

In this experiment, the competing enantioselective conversion (CEC) method was used to react an unknown secondary alcohol, which is either the R- or the S- enantiomer, with S-HBTM and R-HBTM separately. Thin-layer chromatography (TLC) was used to determine which reaction was faster. If the S-HBTM was faster, then the unknown secondary alcohol had the R configuration. If the R-HBTM was faster, then the unknown secondary alcohol had the S configuration. 1H NMR was used to determine the molecular…

Each intermediate product, including N-acetyl-p-toluidine, p-acetamidobenzoic acid, and p-aminobenzoic acid, was checked for yield, presence, and purity through weighing, taking IR and NMR spectrums, and determining the melting point. Thin Layer Chromatography was used to ensure the completion of the final reaction from p-aminobenzoic acid to benzocaine. The yield of the first step from p-toluidine to N-acetyl-p-toluidine was 91.9%. The yield of the second step from N-acetyl-p-toluidine to…

have to make sure their products are safe to sell to the candies to the public. The FDA has to approve of the quality and the “construction” of the candies before they are sold to the public. Basic chromatography is used to enhance the rich and vibrant colors of the shells of the M&Ms. Basic chromatography is also used to make the colors in candy canes more vibrant as food coloring alone won’t be as affective. Therefore, science plays a major role in the mass production of M&Ms and candy…

2). HEROIN: Heroin is formed by conversion of morphine which does not require complex equipmentation but more elaborate chemical operation under which morphine is refluxed with acetic anhydride for about 5 hours results into the formation of Diacetyl morphine, i.e., heroin. It is seperated when the solution gets neutralize with sodium carbonate or washing soda in which heroin gets separated as a precipitate. It is collected and purified by treating it with hydrochloric acid by which Heroin…

information about energy drinks at a college campus level. The different caffeine levels of either the energy drinks or the other caffeinated beverages will be determined qualitatively and quantitatively using two laboratory techniques: extraction or thin-layer…

During scorching summers, as the temperature & humidity gets increased oil granules are produced making your skin oily. Summer skin care tips for oily skin helps in reducing the oil and brighten the complexion to give you a natural glow. There are few pros and cons of having oily skin; Pros: Having oily skin you can enjoy a longer period of younger looking skin Wrinkle-free skin No problem of early aging signs Cons: Excessive Shine Meltdown of Makeup Breakout for acne and pimples But instead,…

Identification and isolation of bioactive principle by coloumn chromatography 9. Discussion and conlcusion 8. RESEARCH METHODOLOGY 1. Hypotheses to be tested: 1. Pharmacological activity of Sesbaniagrandiflora through invitro and invivo antioxidant mechanism 2. Antidiabetic activity of Sesbaniagrandiflora through hypoglycemic…

6. SUMMARY AND CONCLUSION 6.1 SUMMARY The current study deals mainly with phytochemical and pharmacological investigation of traditionally used medicinal plant Enicostemma littorale Blume. A perusal of the literature revealed that only fragmental information was available on this plant species regarding cytoprotective potential and immunohistochemistry. In addition the exact molecular mechanism of action of this plant species active compound swertiamarin is unknown and remote. Hence this study…