Thin layer chromatography

have anti-tumor property. With two undergraduate students that I am training, we purified the rice wine and the fermentation product (acquired using the fermentation starter used at the winery) and performed thin-layer chromatography, high-performance liquid chromatography, and gas chromatography-mass spectrometry to calculate the concentration of farnesol. Future experiments are planned to isolate the farnesol using monoclonal antibody and to perform tissue culture to verify the effects of…

The only solid in the residue that undergoes sublimation is caffeine.(1) In order to determine if our procedure worked and caffeine was isolated we used thin layer chromatography (TLC) to compare the extracted material to a standard solution of pure caffeine. We dissolved the sublimed caffeine in 3 mL of methylene chloride in a clean vial and then swirl the residue in the flask with 3 mL of methylene chloride.…

The purpose of this lab was to use NMR, Recrystallization, TLC, and Melting point to separate and identify the components of a given unknown mixture. The first step of the experiment was to use the two NMR spectra given to identify the major and minor components of the unknown mixture. When an external magnetic field is applied, there is a quantized interaction between the magnetic field itself and the magnetic moment of the proton. Thus, the magnetic moment of the proton either applies with or…

Xanthophylls, which are more polar due to their hydroxyl group, are strongly attracted to Alumina, which would be eluted after Lycopene band. The column should be covered by aluminum foil to prevent Isomerisation of Lycopene by light. A clear orange layer of Lycopene band formed at the beginning of the separation; however there were not enough pigments for collection. The band became more blurred when it reached the bottom. This was due to small amount of pigment obtained during filtration…

2.1 Ageratum conyzoides Ageratum is derived from Greek word, which is “a geras” meaning non-aging, referring to the longevity of the flowers or the whole plant. The word “conyzoides” is derived from word “konyz” meaning resembles (M.X et al., 2016). Ageratum conyzoides is belongs to the Asteraceae family. Plant species in this family are herbaceous plants which trees and shrubs are rare. This plant is very common in West Africa and Asia. It has softly hairy stems and leaves, erect and branched…

tea bags. The tea bad was first brewed and filtered. After brewing and filtering, the caffeine was extracted by using a separatory funnel. Through the separtory funnel, a layer of organic layer formed. This layer was the caffeine sample. In order to test the purity of the organic layer, the procedures for thin layer chromatography…

acetate fraction, 4-5 drops of Hager’s reagent (saturated solution of picric acid) was added and observed for the presence of yellow precipitate formation. Vinblastine and methanol was used as positive and negative control respectively. 4.5 Thin Layer chromatography…

My plan includes functional group conversions of a benzyl ester Knorr pyrrole using various organic reactions (Figure 3), while using mass spectroscopy (MS), thin-layer chromatography (TLC) and proton nuclear magnetic resonance (HNMR) to track the development of DHP. After the coupling of DHP into the tripyrrole, I plan on using various starting materials to adjust the functional groups to find the most suitable variant…

(II, 2-n-butyl-4-chloro-1-[2- (1H-tetrazol-5 yl) biphenyl- 4-yl) methyl] imidazole-5- carboxyl acid)[1]. The determination of Losartan has been carried out in tablets by HPLC, capillary electrophoresis and super-critical fluid chromatography[2,3], in urine by gas chromatography- mass spectrometry[4] and, simultaneously with its active metabolite in biological fluids, by HPLC[5- 10]. Amlodipine Besylate (ADB) )[FIG.1.B], chemically,…

瀀瀀ract The purpose of Isolation of Bixin from Annatto Seeds using Column Chromatography was to separate and purify macroscopic amounts of pure bixin compound from annatto seed ex-tract.1 A mixture of bixin, methylbixin, and norbixin were extracted from annatto seeds. Bixin was then isolated from the mixture through the use of the column chromatography technique. The isolated bixin was characterized through Thin Layer Chromatography (TLC) analysis, which displayed an Rf value of 0.317, which…