Thin layer chromatography

acid, and sodium hypochlorite were added in sulfate is added to remove excess water from the hexane layer that is collected using the separatory funnel.6 To determine the success of the experiment, percent yield was used to determine how much product was recovered, and Thin Layer Chromatography was used throughout the experiment to monitor the reaction process.6 The…

was set aside to be later used in the thin-layer chromatography plate (Setzer, W., 2008). The remaining four-fifths were put back into the separatory funnel to be extracted three times with 5% NaOH in order to deprotonate the solution, which converts it into a salt. Since eugenol is slightly acidic, it can be deprotonated with NaOH. The other components are not acidic and will remain soluble in dichloromethane since they will not deprotonate. The aqueous layers were washed once more with…

Calculations- Representative Fraction (RF): 0.92cm / 8.95cm = 0.10 Limitations- Ruler: +/- 0.05 cm One possible limitation for this experiment was the contamination of the chromatography paper by foreign amino acids. Any amino acids that our gloves came in contact with throughout the lab transfered over to the chromatography paper and appeared as purple spots, mostly on the border of it. Conclusion- The amino acids in unknown mixture A were Glycine and Valine. The amino acids in unknown…

chloride was mixed in, 25 milliliters of ethyl acetate was used to separate the caffeine from the organic layer. Ethyl acetate is a polar compound that is used to attracted caffeine from tea leaves or coffee beans. Because caffeine is polar, it easily attracts to ethyl acetate. As two layers became visible, the top layer containing the caffeine and the bottom one the organic layer, the top layer was removed by a pipet. The solution that contained the caffeine was place in a clean 150 milliliter…

Examples of some preparative chromatographic techniques include: • Thin Layer Chromatography (TLC) • Column Chromatography (CC) PREPARATIVE THIN-LAYER CHROMATOGRAPHY A method for rapid qualitative analysis, TLC here allows components of a mixture to be separated easily. The solid phase is made of a thin layer of silica mounted on a solid support, called the TLC plate. The properties of the silica and the properties of the components are…

aeruginosa than Saccharomyces cerevisiae. Under this study, after centrigugation of crude extract,the pellets were suspended in a 40 mL of dichloromethane in a separating funnel and shaken vigorously and the allowed surfactant recovered in the organic layer at…

Caffeine from Tealeaves Rosheen Villaraza, Dana Young, Dana Yu*, and Ray Zafra Department of Biological Studies, University of Santo Tomas, Manila, Philippines Abstract Experiment 3 highlights the separation of caffeine from tea leaves and using chromatography for testing its purity. Extraction is a method of separating the desired from the undesired substance in a mixture through the solubility properties of the substances. In the experimental extraction, Camellia sinensis was used and…

small-scale recrystallization is performed on the benzanilide-thiourea crude mixture. (c) Filtration of purified crystals. (d)Product recovery is calculated and measured. (e) In order to perform thin layer chromatography on the purified crystals, an effective eluent must be selected. (f) Thin layer chromatography is performed to assess the purity of the recrystallized product. (g) Melting point determination is performed in order to assess the purity of the recrystallized product.…

Despite the practical and informative nature of the Williamson ether synthesis of 2-bromo-4-methoxy-phenoxy benzyl ether. This reaction was not conducted due to an error made in which the initial product was thrown away instead the actual second phase of this synthesis was the Williamson Ether synthesis of 4-methoxy-phenoxy benzyl ether. Figure 4. Williamson Ether Synthesis 4-methoxy-phenoxy benzyl ether ____________________________________¬___ In spite of this outcome there was still much…

uses for this compound in medicine if it can be separated effectively from tea granules. Caffeine acts as a stimulant, making humans more mentally alert. Two methods used to separate caffeine from tea granules are Thin Layer Chromatography and High Performance Counter Current Chromatography. In TLC, a slide of glass coated in a substance can act as the stationary phase, serving as a bed for the mobile phase to move along. Samples are applied at a certain height to be carried and separated.…