Thin layer chromatography

“DEVELOPMENT AND VALIDATION OF STABILITY INDICATING METHOD FOR SIMULTANEOUS ESTIMATION OF CEFTRIAXONE AND TAZOBACTAM INJECTION USING RP-UPLC METHOD” 2.1 OBJECTIVE To develop and Validate Stability Indicating Method for simultaneous estimation of Ceftriaxone and Tazobactam in their combined dosage form by RP-UPLC method in various stress condition like, alkaline, acidic, oxidative, thermal and photo degradation. 2.4 METHODOLOGY 2.4.1 Chromatographic Condition Column Separation was achieved by…

Chromatography is a method of separation in which the components of a substance are separated and distributed between two phases, which are the mobile phases and stationary phase (1). There are multiple types of chromatograph, which includes paper chromatography, column chromatograph, retention chromatography, gas chromatography, liquid chromatography etc. Each technique has its own strengths and weaknesses, but all are useful in getting separations of different components (8). Since many…

High Performance Liquid Chromatography Lab Abstract High performance liquid chromatography or HPLC is the process in which mixtures are physically separated according to their polarity. The lab conducted, the separation of dyes in grape Kool-Aid, demonstrated the concept. This was done to display how different alcohols and their concentrations have different polarities, in which ties into HPLC. The lab consists of testing Kool-Aid, water, and different concentrations of methanol and…

Introduction: Centrifugation is a process used to separate or concentrate materials suspended in a liquid medium. It is a method used to separate molecules under the centrifugation field based on their sedimentation rate with the help of centrifugal force. It is also used to measure the physical properties of a molecule like molecular weight, density, shape, etc. If it is used for the separation of one type of materials from others is termed as preparative centrifugation and when used for…

Introduction Chromatographic process is a separation technique which has to do with the mass transfer of test sample between a stationary and mobile phase. HPLC (High Performace Liqud Chromatography) is a vast system which involves the use of a mobile and stationary phase to separate materials. The stationary phase is usually a column packed with solids (usually silica gel) while the mobile phase is usually a solvent or a mixture of various solvents. The mobile phase usually, is the carrier of…

The invention relates to formulation of Ketorolac Tromethamine and Phenylephrine Hydrochloride immediate release tablet having less disintegration time. HPLC method was developed and validated for determination of content of Ketorolac Tromethamine and Phenylephrine Hydrochloride in immediate release tablet. UV spectrophotometric method was developed and validated to study drug release profile of Ketorolac Tromethamine and Phenylephrine Hydrochloride in immediate release tablet. Background:…

cooling the test tubes under tap water, 1.0 ml of distilled water and 5.0 ml of the mixture of n-butanol and pyridine (15: 1, v/v) was added and shaken vigorously. Centrifuged the test tubes at 4000 rpm for 10 minutes and the absorbance of the upper layer were measured at 532 nm. Standard malondialdehyde was taken as the standard and was processed in the same manner. The level of lipid peroxide was expressed as µmoles of MDA formed /g wet…

prioritizing the continued security of the power grid should be at the top of the list. There are many different options that can improve security levels of both a physical and logical nature, such as disk drive encryption, digital certificates, Transport Layer Security (TLS), and Virtual Private Networks. Recommendation: Implement Full Disk Encryption Full disk encryption is the ability to convert data into unreadable code that can only be deciphered by authorized people. The encryption can…

would not have done anything to stop this type of compromise. The connection to server uses HTTPS pages that could use one of two secure protocols to encrypt communications (What is HTTPS, n.d.). The first is SSL (Secure Sockets Layer) and the second is TLS (Transport Layer Security) and both use what is known as an asymmetric Public Key Infrastructure (PKI) system (What is HTTPS, n.d.). With an asymmetric system it uses two keys to encrypt communications, a public key and a private key (What…

The comparison of lining and interlining materials are given in Table 13.2 Table 13.2 Comparison of lining and interlining materials Sl. No Lining Interlining 1 It is used inside of garments or garment components. It is used between two layers of fabric. 2 It is attached by sewing. It is attached by sewing or applying heat and pressure. 3 Finishing is not necessary. Sometimes finishing is necessary to improve its properties. E.g. shrink resist finish. Crease resists finish. 4 No coating is used…