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    proteins, and amino acids.2 Chromatography is the process of separating a mixture, and has many different applications throughout the world. There are a variety of ways to conduct chromatography including column chromatography, paper chromatography, and thin layer chromatography. The particular experiment conducted and discussed is paper chromatography. This experiment will use chromatography to test and identify unknown pen inks based on tests done on known pen inks. Understanding the history…

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    Abstract In this paper CuO-NH-NH2 nanoparticle was synthesized and used to remove anionic dyes from single and binary systems. The scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), Energy-dispersive X-ray spectroscopy (EDAX) and X-ray diffraction (XRD) were used to characterize the nanoparticle. Direct Red 80 (DR80) and Direct Green 6 (DG6) were used as anionic dyes. The effect of adsorbent dosage, dye concentration and pH on dye removal was evaluated. Kinetic…

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    Chromatography Lab

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    Chromatography Abstract: The purpose of this lab is to use chromatography to separate and purify organic compounds. There are many different types of chromatography but most depend on the principle of adsorption. The two important components of chromatography are the adsorbent and the eluent. Adsorbents are usually solid materials that will attract and adsorb the materials to be separated. The eluent is the solvent, which carries the materials to be separated through the adsorbent. After being…

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    Chromatography Lab Report

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    This disagreement was about the pigments located in the stems and veins of a plant and the pigments located in the green portion of a leaf. Jan believed that only the stems and veins contained all of the important pigments except for chlorophyll. She believed that the green portion of the leaf would not contain all these other pigments. Mark, on the other hand, believed that the same pigments were also located in the green portion even though they were not visible. In order to determine who was…

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    Thin-Layer Chromatography Lab 11/12/14 Beginning Question To what extent does the polarity of a substance affect the distance it travels during TLC using water as the solvent? Safety Concerns The substances used during the TLC process could possibly be dangerous, so it would be best to avoid getting them on the hands. If one were to accidentally spill the water used in the process onto the floor, a slipping hazard could occur. Data Dye Solvent Front (cm) Spot (cm) R…

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    The goal of this experiment was to use the technique of thin layer chromatography (TLC) to identify the active analgesic ingredient of Advil and monitor the transesterification of methyl salicylate from acetylsalicylic acid. The synthesized methyl salicylate was then purified using acid-base chemistry and analyzed using H NMR spectroscopy. Identification of a proper mobile phase to separate common analgesics used in over-the-counter pain relievers was essential in being able to determine the…

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    Chapter Three - Conclusions 3.1 Conclusions This research aimed to verify the synthesis protocol for HPA-12 and analogues from aldehydes of Greatrex et al. (2014). Limited success in synthesising required quantities had a negative impact on the outcomes of this research, and no reasons were identified for the low yields that were obtained. Additional time, or resolution of issues resulting in low yields, would have allowed additional research and confirmation of the synthesis protocol. Long…

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    Tlc Lab Report

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    TLC and HPTLC Introductions Thin layer chromatography (TLC) is derived from liquid chromatography where liquid serves as mobile phase and thin layer of substance on a flat late surface serves as stationary phase. The thin layer of materials act as sorbent while the mobile phase known as solvent. Solvent will send the solutes through the sorbent depends on the strength of the mobile phase as it dissolved the solute and send them up the plate against gravity ,and resistance of sorbent…

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    Separation through Ion Exchange Chromatography Introduction Amino acids are important molecules that serve as building blocks for all proteins that are made in every organism. Although there are millions of different proteins that can be made by all living things combined, all proteins are derived from a base of 20 amino acids that are combined together in different ways to create the diversity of life that is on Earth. Humans cannot produce all of the amino acids needed for protein synthesis,…

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    To identify the unknown chemical mixture (#757), a TLC analysis was conducted using three standards (aspirin, acetaminophen, and caffeine). A TLC plate was prepared by drawing an origin line and solvent front line with a pencil. Three spot were labeled A, B, and C. A sample of each standard was spotted on the analytical TLC plate approximately 1cm from the bottom of the plate. It was then enclosed in a jar filled with 2ml of 95% ethyl acetate- 5% acetic acid. (Another laboratory group conducted…

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