The Tubes

The purpose of the lab was to determine the stability constant of the complex ion Thiocyanatoiron[1], Fe(SCN)+2. This was done through the lab technique, spectrophotometry. The main chemical equation focused on for the experiment was [Fe(H2O)6]+3(aq) + SCN-(aq)  [Fe(H2O)5(SCN)]+2(aq) + H2O[2]. However, because the concentration of water in the dilute aqueous solution is constant it can be written in the simple form of Fe+3(aq) + SCN-(aq)  Fe(SCN)+2(aq)[2]. In the experiment the stability…

Introduction: Bacteria create an enormous domain of prokaryote microorganisms that are diverse and abundant in most habitats on Earth. In fact, bacterial cells drastically outnumber somatic cells just on the external part of the human body compared to somatic cells within the whole body of a single individual. Bacteria are found just about anywhere on earth and are usually alongside other microorganisms including: mold spores or yeast cells, creating some difficulty when trying to distinguish…

Abstract Many number of medicines, like growth hormones and insulin, are a possibility because of techniques like transformation of genetically engineered bacteria. Although, manipulating bacterial gene expression has many great implications, it can also be quite harmful. By manipulating gene expression of bacteria, such as Escherichia coli, scientists now have the capacity to help bacteria survive changes in their environment and provide an evolutionary advantage. The present study was…

The Effects of pGLO Plasmid Injection on Prokaryotic Gene Expression. By Kush Patel 3rd Period, AP Biology, February 12, 2024. Lab Partners: What is the difference? Brody Bonsor Andrew Zhang Marianne Baquero Clarie Gabrielson Abstract: In the pGLO lab, E. coli bacteria were genetically modified using a plasmid that contained three key genes: araC, GFP, and ampicillin resistance. Through a process of heat shock and incubation, the pGLO plasmid was successfully introduced…

Leave at room temperature for 2 minutes Add 1 ul of superscript II RT to each tube. Mix thoroughly and incubate at 25 Cfor 10 min Incubate tubes a 42Cfor 50 min. Then heat tubes at 70Cfor 15 min. Cool on ice Add 1 ul RNase H and incubate at 37Cfor 20 min Store the first strand of cDNA at -20Cfor real-time PCR Real-Time PCR Normalize the primer concentrations and mix gene-specific…

“A catheter is a tube that can be inserted in a person for drainage, they are generally used with urinary tract or kidney problems” (Pavao 1). “The first Catheter was a hard metal tube” (Pavao 1). “He created a pain-free model by making a thin, flexible tube” (Pavao 1). “This tube could move as needed, in order to cause less pain” (Pavao 1). “Modern doctors still use the tube Franklin design” (Pavao 1). The final one of his inventions that showed…

Results and Discussion Rather than using organic solvent extraction, CO2 extraction was used to separate D-limonene, a component of orange oil, from orange rinds. CO2 extraction is considered a “green” experiment because it is nonflammable, relatively nontoxic, readily available, and environmentally benign. Considering that there is not a high concentration of limonene in the rinds, the experiment was successful and yielded 1.04 percent limonene relative to the mass of the rinds. The 13C…

Protein Purification III Parts 09/15/2015, 09/22/2015, 09/29/2015 Kaisha Butz Lab Partner: Ryan Van Abstract The lab was comprised of three parts, the purpose of which was to separate a protein mix and analyze the results. The first part was separating the proteins; the second part was reacting the proteins with an enzyme, and the third part was analyzing each fraction from Part I to see if separation was successful. It was hypothesized that out of the four proteins…

the measuring cylinder, thus the volume of hydrogen peroxide could have been below or above the 5mL mark due to parallax. This would affect substrate concentration if volumes in test tubes were inconsistent, thus some test tubes might have less or more than 5mL of hydrogen peroxide, reducing precision. If a test tube contained more than 5mL, the substrate concentration would be higher, thus may increase reaction rate (Sam Adam-Day, 2014), and vice versa. Increase sample…

occurred: the pH paper was accidentally placed in the test tube in step ten, and more than the recommended water input was added to the test tube in step eleven. More water was added in step eleven because the solution was too cloudy. The results of Part B were interesting because it was expected that there could only be calcium or barium present in the solution, but both were confirmed present. A precipitate formed in both of the tubes that were needed to be used to confirm the presence of…