The Medium

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    paragallinarum reference strains (HP1, HP 90 and HP144) and two Australian Av. paragallinarum reference strains (HP14 and HP60) were selected for growth curve construction. Three broths were tested for their suitability of growth, Haemophilus Test Medium Broth (TMB), Cation-adjusted Mueller-Hinton Broth (CAMHB) with supplements and Veterinary Fastidious Media (VFM). TMB was prepared with 1% Biosate Peptone (BD), 1% sodium chloride (Merck Millipore, Australia), 0.1% starch (Merck Millipore), 0.1%…

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    Gram Negative Bacteria

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    The purpose of the unknown experiment is to understand and identify a gram positive and a gram negative. Bacteria can be very helpful in clinical uses and many pharmaceutical drugs are based on the organism. Knowing how a bacterium works and how it is structured can be crucial to determine how it affects each individual. Several biochemical tests were performing to separate and determine the identities of the microorganisms. Broth tube number 31 containing unknown microorganisms. One of the…

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    I look up to the sky and see millions of stars, and I wonder to myself. What makes them light up so bright, and what makes them a star? What does it take to create a star; they just don’t pop out and shine bright. I want to know the process, and how long it takes for a star to be born. I am also curious to know if the star can die. I plan on finding the answer. I believe that they are created by gases and that they die when they use up all of their gases. Stars have had an impact in our…

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    As people decided to sending human into deep space to explore unknown feature of the universe, the environment outside the earth is a big uncertainty with factors that we can’t fully understand yet. Besides the cost and the time we spend during long space travel, stay in the cold, vacuum universe would let astronauts experience different kind of radiation. These radiation may be some ionized particles as electromagnetic waves traveling with high speed and energy. They could come from nearby…

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    Transformation of pGlO plasmid to an E.coli bacteria cell We pipetted 250 ul of CaCl2 transformation solution to into two separate tubes. Next, we used a sterile loop to transfer two to four larger bacteria colonies from an E.coli plate into each of the two tubes. The bacteria was observed under UV light to make initial observations. We pipetted 10 ul of pGLO plasmid (0.08 ug/ul) into one of the tubes. Both tubes were incubated on ice for ten minutes. After ten minutes of incubation, the tubes…

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    6. Three Nutrient Agar Plate (NAP) were contaminated as follow to illustrate that there are microorganism all around us and to demonstrate the necessity for proper aseptic technique. a) The lid of the first agar plate was removed and the exposed agar was placed in the building on the laboratory rack for 3 hours. The lid was replaced and labelled as “air”. It was then incubated at room temperature. b) A second Petri plate was divided in half using a marker. Two cotton sterile swab were moisten in…

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    For this experiment, unknown sample #12 was used. The first step in identifying any unknown bacterium is to grow a culture on a nutrient agar plate. This bacterium was also plated on a MAC plate, which is both selective for gram-negative bacteria and differential as well. On the NA plate, bright red colonies were present; on the MAC plate there was bacterial growth as well, but no color change was observed. This lack of color change on the MAC plate indicated that the bacteria did not ferment…

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    In the short passage of The Medium is the Message by Marshall McLuhan, he explains his views on the transition from verbal communication to writing words down on a paper, and also the constant advancement of technology. McLuhan proposed that writing words on a paper led to inventions such as book, roads and more. At the same time, writing caused Western society to live in a world of invisible lines. He emphasized that alphabet had no true meaning until lines were added to it. Also that before…

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    Small and Medium Enterprises (SMEs) are the businesses, which employ less than 250 persons. Though they are largely unstructured businesses, they interestingly, account for the lion’s share of the workforce in virtually every economy. To a large extent, they are usually sole-proprietorship businesses, especially at the micro and small levels. Due to their small sizes, the large labour they employ, the ease of starting up and speed of adaptation to emerging trends and consumer tastes, SMEs…

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    Does the medium prompt the advancement of holiness? Does the piece in question provide insight to the human condition, giving the viewer a better understanding of themselves? Consider an abstract piece that represents humanities hopelessness can better be understood…

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