The Dish

Oral Microbiome 10: Reflection 3 One a daily basis the human mouth can come in contact with many different materials and organisms. “The human mouth is home to billions of individual microorganisms, including viruses, protozoa, fungi, archaea, and bacteria” (University of Minnesota Department of Biology Teaching and Learning, 2016, p. 21). In specific, during this lab we are looking at Streptococcus mutans (S. mutans) and Lactobacilli. In studies, it has been shown that yogurt has helped…

Objective I. To learn how to inoculate the nutrient broth properly in preventing contamination to the nutrient broth and the culture Escherichia Coli. This is done by sterilized the loop and sterilized the neck of the tub with heat from before use and before put the cape on. II. To learn how to do a simple streak of an E. coli culture on a nutrient agar plate with one stroke from start to end like a “Z”. III. To learn how to do a complex streak where we grow E. coli culture on the nutrient…

Vocabulary 1. Inoculum: a material used for inoculation. 2. Loop (the tool): A wire made into a loop to assist in picking up inoculum from a culture of microorganisms. The loop is also used in the process of cultivating microbes by transferring inoculum for streaking. 3. Agar vs Blood Agar: a. Agar: a growth medium made with agar and other nutrients. The agar allows microorganisms such as fungi and bacteria to be cultured. b. Blood Agar: Blood agar is used for the purpose of cultivation and…

Procedure 1. Find the materials. 2. Take your four postit notes and label them tap water, well water, spring water, or distilled water. 3. place the Post-it notes on a flat surface. 4. Fill each jar with one cup of one type of water. 5. Place the jars on top of the correct Post-it note. 6. Take one small piece of duct tape and place the top line of the tape at the top water line for each jar. 7. Make a table like the one shown. 8. Put however much soap you want in A soap container. 9. Bill the…

In this lab, we took four separate onion bulbs, labeled them A,B,C, and D, and put them in a beaker filled with water. We recorded the growth in number of roots and their average length for days one, two, and five. Then we put Bulbs B, C, and D in beakers with different amounts of caffeine and left Bulb A in water. We then recorded the number of roots and their average length on days two, three, and four. After that, we took and onion root form Bulbs A, B, C, and D, stained them, and looked for…

Blood Culture Contamination For the purpose of this research, Archbold Medical Center Laboratory is the place I chose to conduct my research. Blood Culture contamination is a big problem not only at Archbold, but at many hospitals. The problem is not only within the laboratory, but from outside sources also. Although contamination is a problem, it can be easily fixed. Whenever a blood culture is ordered by a physician, we can assume the patient is experiencing some type of bacterial infection…

To begin, a streak was performed on two TSA plates and a blood agar plate using the three-sector streak technique from the unknown culture tube to isolate the two unknown bacterial colonies. The plates were then incubated for 12 hours at 37 ̊C. The blood agar plate was used to create a greater contrast in color to help distinguish between bacterial colonies by size and color. Two distinct colonies could be seen growing on the blood agar plate shown in figure 1. The smaller, opaque colored…

In “The Existential Anguish of the Tattoo”, I found many interesting ideas, but I thought there was a hint of biases in some cases. Overall, I thought it was a superb article even though there were some things I did not agree with. It was beautifully written and the “Existential Anguish” was apparent as you read on. It gave me a great sense of nostalgia and wonder; it makes you think quite deeply. It was eloquently written and its subtle use of pathos was lovely. Dan Brooks’ use of metaphors and…

figures out later that her pickle dish was shattered, the reader finds out how special the dish was to her. She received the pickle dish as a wedding gift from her Aunt Philura Maple, leaving the dish on top of a shelf, never getting it down “[ex]cept for spring cleaning, lift[ing] it with [her] own hands” (Wharton 69). She never brings down her dish, “not even when the minster come[s] to dinner, or [her] Aunt Martha Pierce” (Wharton 69). It is obvious that the pickle dish means so much to her…

2.5. Morphological Identification/Evaluations of Entomopathogenic Fungi The six isolates of fungal entomopathogens were provided by Department of Entomology, Penn State USA. Preliminary morphological identifications of fungal entomopathogens such as color of colony, mycelial characteristics and spores from all six isolates were done at microscopic level. 2.6. Molecular Characterizations of Entomopathogenic Fungi Cultures of Metarhizium fungus were grown in nutrient broth (Bacto, Australia) in…