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Immunocytochemistry (ICC), which this lab is based on, is commonly used as a laboratory technique to anatomically observe the location of a specific protein or antigen in cells by the use of a specific primary antibody that binds to it. A staining reagent or chromagen is used to help detect certain proteins used in this experiment, called 3,3'-Diaminobenzidine (DAB). 3,3'-Diaminobenzidine (DAB) DAB, a derivative of benzene, is an organic compound, which is both chemically and thermodynamically…

To do so, two cells with DAPI (blue) and added Venus fluorescent proteins (Green) were tagged. These proteins are only detectable when there is a dimerization of Hpo. The first cell, which just included the Hpo kinase- dead proteins (HpoVc-VN), exhibited no green fluorescence meaning that Hpo did not dimerize (Figure 4A). The second cell,…

On one hand, there are many ways in which Facebook itself limits the ways in which community can represent itself and, on the other hand, people do temper themselves in many actions when creating Facebook–selves. Firstly, some users do not represent solely their real–selves. Many users shape their online profiles based on their false– and ideal–selves for various reasons, such as: exploration and deception, self–promotion, social pressures, shame of the real–self, gaining popularity (Gil–Or…

Women Fighting for Equality in Their Devilish Ways How can a woman become devilish? According to the book Shame the Devil, by Debra Brenegan, Sara Payson Willis had the devils wickedness in her because she “…wiggled during prayers, interrupted conversations, asked too many questions…” (1) Sara did not possess the feminine qualities like modesty, humility, piety, weakness and submissiveness. She was strong, independent and outspoken and these qualities made her a devilish woman in the 1800s.…

is displayed on their main profile page. Moreno, Swanson, Royer, and Roberts (2011) stated that people judged newly acquainted friends by their uploaded and tagged pictures online. Tagging pictures is a Facebook function where the program uses facial recognition as a way to identify the individuals within the picture. Once a picture is tagged that image will show up on an individual’s profile page. Moreno et al. (2011) further claim the well-known phrase “a picture is worth a thousand words”…

8050 North 9th Ave Olive Rd. Brisbane, CA 94005 August 05, 2017 Hi Whitney, I hope all is well in your new city. Everything here is passing by so quickly, and the summer months are almost gone. It’s only been a little while since you left, but it everything already feels different. Even though it’s super sad that you don’t live just a bus ride away anymore, I really do hope that your new life is going well. It’s so exciting that you’re moved in and ready to start college! It must be so…

Baby years and family history On my mom's side family means everything. We are constantly going to family gatherings. My grandparents had 5 kids so counting their spouses I have 6 aunts and uncles and 7 younger first cousins. There is lots of drama and no privacy. My dad's side is a different story he is an only child and his mom has remarried many times so we never really have a grandpa on his side. We go and see my granny but that's about the extent of going to see my dad's side. My…

she’s a bit different from the rest. This introverted nine year old refused to hang out with the other children. When playing tag, she simply wouldn’t budge. ‘Margot stood alone.’ (67) She was constantly alone, not wanting to socialize. ‘If they tagged her and ran, she stood blinking after them…

Possibility Looms on Johnny Manziel Getting Dumped By the Cleveland Browns It was at the headquarters of Cleveland Browns that Johnny Manziel necessarily missed a particular concussion checkup and this happened just a single day after he was spotted in Las Vegas. According to the post-concussion protocol of NFL, Manziel, aged twenty three (23) years, has primarily been ruled out of the game against the Pittsburgh Steelers that was scheduled to take place on Sunday. A section of employees and a…

“We’ll need to head out through the other end of the cargo bay where we’re going to be decontaminated and have our suits disposed of.” “Yes, of course.” They made their way to the other end of the cargo hold moving cautiously through the rows of body bags. Then, unexpectedly, Commander Brayzack stopped. “Wait!” “What is it, sir?” “I need to see that database again.” Back at the computer, Brayzack began typing one more name into the search box, Enders…