The optimized PF0 encapsulated hybrid NCs formulation was achieved by a two-step process (Fig 1) using nanoprecipitation and subsequently coating with chitosan. In Step-I, the nanoprecipitation technique was used to prepare PF0 loaded BSA NCs (PF0-ANC). In this nanoprecipitation technique, acetone was used as a desolvent [40]. Varying concentrations of PF0 solutions (125µM - 375µM) and BSA solution (156.25µM – 468.75µM) (Table 1) were mixed in a glass vial. The PF0 BSA solution was then added…