Succinate dehydrogenase

this lab, the cells from a cauliflower were studied with the purpose to separate nuclei and mitochondria content from a solution. To achieve the results, the cauliflower solution had to go through differential centrifugation, microscopy, and succinate dehydrogenase assay. For the fist part of the lab density played a key role in the separation of the cells content because mitochondria and nuclei are very different in size, mitochondria is approximately ten times smaller than nuclei. So it was…

succinyl-CoA synthetase, succinate dehydrogenase, fumarase, malate dehydrogenase, and citrate synthase. The Cori Cycle has many parts to it ultimately leading to the breakdown of glucose and the diffusion of lactate with the liver. In the Cori cycle there is the conversion of glucose to lactate through anaerobic glycolysis in muscle cells, the diffusion of lactate from muscle cells into the bloodstream then being transported to the liver. To the conversion of lactate to glucose through…

Hypothesis: Aerobic Respiration: If a phosphate buffer, dichlorophenol-indophenol and succinate are added to a mitochondrial extract then the succinate molecules will bind to an enzyme complex (succinate dehydrogenase). Because the enzyme and FAD are a part of the same complex, initiating succinate oxidation, this reaction reduces FAD to FADH2. Thus the oxidation of succinate to furmarate. Anaerobic Respiration: If a solution of yeast and glucose is combined and placed in different incubation…

Oxaloacetate, found in the mitochondrion, is a four-carbon molecule. In this project we performed a Bradford Assay and an SDH (Succinate Dehydrogenase) Assay. The goal of this project was to work with enzymes to gain a better understanding of how enzymes function and how they are impacted with the introduction of a variety of factors. A significant part of the experiment was determining the protein concentration of the prepared mitochondrial fraction which will be further explained. The reason…

multi enzyme complex pyruvate dehydrogenase to produce acetyl CoA. 7, 8, 10. The citric acid cycle also provides carbon skeletons for the biosynthesis of amino acids, fatty acids, carbohydrates, cholesterol, haeme and other molecules that are vital for survival. 1, 5, 6,…

Generates the majority of adenosine triphosphate known as ATP, for energy. The pellet will be formed after the 2nd centrifugation; the absorbance measured of the mitochondria will be low. Succinate dehydrogenase (SDH) is an enzyme located in the mitochondria membrane. FAD is present, it then turns the succinate to cause cellular respiration. SDH will replace with DCIP, which is an artificial electron acceptor. DCIP is used to measure mitochondrial activity in the spectrometer. DCIP is a redox…

Fish have a unique position with respect to their taxonomic and phylogenic status among vertebrates and occupy an important place in vertebrate’s genealogy. Most fish are ectothermic ("cold-blooded"), allowing their body temperatures to vary (1 – 2 ºC) as ambient temperatures change. Majority of siluroid fish are economically important for human consumption. Protein and vitamins content in fish flesh varies from high to moderate and make it as rich source of protein diet. Fishes of siluroid…

metabolism is associated with increase in glycolytic pathways by what is known as the Warburg effect. The lack of oxygen due to decreased vascularization makes the mutated cells rely on an anaerobic production of lactate from pyruvate by lactate dehydrogenase accompanied by the oxidation of NADH to NAD+, with a net production of 2 ATP as their main energy source. The NAD+ produced is used as an electron acceptor by the mitochondrial transport chain requiring a stable and high concentration…

The laws of heredity discovered by G. Mendel are similar for all complex life creatures and he concluded that genes come in pairs one from each parent inherited as distinct units. Also, the mathematical patterns of inheritance as well as genes segregation and their appearance in the offspring as dominant or recessive traits were determined during his genetic experiments with pea plants and outlined three laws oh inheritance. First, the Law of Dominance is expressed where one allele dominates…

The racehorses averaged the age of five and the regular riding horses averaged the age of thirteen. The researchers examined the plasma malate, M/L ratio, and lactate dehydrogenase activities to figure out the energy metabolism in the horses. Plasma malate is incorporated in the ATP and NADH generation, but lactate dehydrogenase can convert NADH to NAD+ for the making of ATP. The increase of plasma M/L ratio can show a heightened metabolism energy in the skeletal muscle and liver of the horse…