Succinate dehydrogenase

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    Succinate Dehydrogenase

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    Hypothesis Experiment I Eukaryotic cells contain several membrane-bound organelles. Plant cells are a specific type of eukaryote that are both aerobic and photosynthetic. Because of this, they contain mitochondria that serve a vital role in aerobic respiration that follows photosynthesis. The enzyme succinate dehydrogenase (SDH) is an enzyme associated with the Citric Acid Cycle that takes place in the mitochondrial matrix. SDH serves as a catalyst for a reaction in which succinate and FAD become fumarate and FADH2. The SDH-FADH2 complex then reduces Ubiquinone in the electron transport chain. SDH can be indirectly measured using the artificial electron acceptor DCIP that is blue in its oxidized state and colorless in its reduced…

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    enzymatic activity of succinate dehydrogenase (SDH) in various solutions, specifically those containing and not containing a reagent. The assigned reagent was malonate, which was previously hypothesized to be an inhibitor of SDH. Bovine liver was manipulated primarily through centrifugation, and mitochondrial fractions were then created to serve as the enzyme-rich solutions the reagent would act upon. To determine the protein concentration of the mitochondrial fraction, the Bradford assay was…

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    Mitochondria Experiment

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    third strand of fly used was the sdhBEY12081 strain (mutant) that had a mutation present on the sdhb gene of the mitochondria. The sdhBEY12081 gene is responsible for producing the enzyme succinate dehydrogenase, which is used in the electron transport chain for ATP production. The results showed that the sdhb (mutant) strain of flies developed the quickest, the wild-type strain was second, while the W501 (hybrid) strain had the slowest developmental time. As regards climbing ability, the…

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    Abstract This experiment studies the rate of activity of succinate dehydrogenase protein derived from the mitochondria of cauliflower cells. Succinate dehydrogenase, once active, is known to reduce the coenzyme flavin adenine dinucleotide (FAD) to FADH2. Succinate dehydrogenase and its coenzyme creates an enzyme complex (E-FADH2) which transfers electrons to coenzyme Q during the Krebs cycle. In this experiment, sodium azide will be added to the mitochondrial solution to prevent the transfer of…

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    Reflection On Loneliness

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    In response to #18 on application I believe myself to be rehabilitated in full. My incident was a time where I made a mistake that I never have before. Since that time, I have set hours to complete all community service and ordered counseling. I started my community service and counseling right away as I know how important it is to complete what is ordered. By December 10, I plan to have all community service completed. I am working at a community tutoring center and a state park in order to…

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    lowers the freezing point of the leftover water in the frog’s blood, which is moved into areas where ice crystals will cause the least damage so the frog is left unharmed. Much like how the wood frog survives cold conditions, the frequent urge to urinate and increased blood sugar in diabetics may have helped some of the Northern Europeans survive the extreme temperatures of the Younger Dryas. Favism, an inherited enzyme deficiency, is the most common enzyme deficiency in the world, and is…

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    macrophages in people who have hemochromatosis surround foreign invaders, and since the bacterium have no iron, they starve to death in the body. People with the disease, although they will die later in life from iron buildup, can survive diseases that are potentially more harmful and survive long enough to pass the mutation on to their children after reproduction. This stronger defense system helps to fight off bacteria and other invaders, and helped many escape the bubonic plague, explaining…

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    Hypothesis: Our reagent, Malonate, is an inhibitor of the mitochondrial enzyme Succinate Dehydrogenase (SDH) that will bind to the active site of the enzyme and result in a decrease of enzyme activity. Justification: Any agent that interferes with enzyme activity is an inhibitor. Inhibitors can affect the catalysis and/or the binding of enzymes to substrate. Malonate is an inhibitor of SDH primarily because of its shape and function, as the shape is similar to that of succinate (Clark, 2009).…

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    Krebs Cycle Metabolism

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    One things importants, the citric acid cycle is an amphibolic pathway. The krebs cycle a role in anabolism. For example the Anaplerotic reaction, these reactions replenish the intermediates of the citric cycle that are removed to serve as biosynthetic precursors. Thry are regulated to keep the level to intermerdiates high enough to support the activity of the citric cycle. And for finally we will talk about the glyoxylate cycle , the glyoxylate cycle catalyzes the net conversion of acetate to…

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    organelle differentiates from one another by its content. In this lab, the cells from a cauliflower were studied with the purpose to separate nuclei and mitochondria content from a solution. To achieve the results, the cauliflower solution had to go through differential centrifugation, microscopy, and succinate dehydrogenase assay. For the fist part of the lab density played a key role in the separation of the cells content because mitochondria and nuclei are very different in size,…

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