several incision and drainage procedures.” Potential Pathogen In the case of the individual that developed an infection after receiving bilateral prosthetic hip replacement, the pathogen that appears to be the most likely culprit is Staphylococcus aureus (S. aureus). The patient likely did not…
which showed antimicrobial activity. Compound 3b has broad spectrum activity against Gram +ve bacteria, Gram -ve bacteria, Yeast and Filamentous Fungus with MICs 150, 150, 75, 312, 625 and 625 µg/mL against (Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 3555, Escherichia coli ATCC 23282 Pseudomonas aeruginosa ATCC 10145, Candida albicans IMRU…
with lethal inflections of methicillin-resistant Staphylococcus aureus, a combo of the standard antibiotic and a small molecule was able to cure the infection. However, mice treated with just with antibiotic continued to suffer with methicillin-resistant Staphylococcus aureus. This experiment found that the small molecules created and given to the first group of mice were able to reverse the drug resistance in methicillin-resistant Staphylococcus aureus, in addition to leaving no harmful side…
Normal flora is the colonisation of human tissues by a diverse mixture of microorganisms. This colonisation begins at birth with pioneer species, depending on physiochemical factors. The normal flora inhibits the skin, oral cavity, upper respiratory tract, gastrointestinal tract, urogenital and conjunctival membranes. They are commensal bacteria; getting shelter and nourishment from the host without causing harm (Gould, 2012). To a healthy individual, most normal flora is harmless, however some…
2.5. ANTIOXIDANT STATUS 2.5.1. TLC - Semi Quantitative DPPH Assay 0.2 % DPPH solution in methanol was prepared and kept in the fridge for further use. The grid space was marked with 1.0 cm2 space on an aluminum based TLC sheet (Merck silica gel 60F254) and a stock solution of all the extracts together with the standard were prepared in methanol. A series of dilutions of the stock together with the standard were prepared ranging from 400 µL to 0.01µL for the last dilution. The grid on the TLC…
infection; then, determine which particular pathogens, or groups of pathogens, are the underlying cause of the infection. In the clinic, infections manifesting from gram-positive cocci are a common and growing problem. Two genera in particular, Staphylococcus and Streptococcus, are responsible for a variety of human infections, hence, it is crucial to learn techniques, which aid in the quick identification of a particular microbe. Applying these techniques in our future careers will…
(Bardiau et al., 2016; Shaheen et al., 2016; Blood et al., 1978). Staphylococcus aureus (S. aureus) is considered the main worldwide causative agent of 40-70% of contagious bovine mastitis (Bardiau et al., 2016; Shaheen et al., 2016). Moreover, S. aureus is a dangerous threat to…
Maryland where I am currently working as an Intramural Research Training Award postbaccalaureate (IRTA) research fellow at the National Institute of Health (NIH) investigating against the common deadly pathogen, MRSA (methicillin resistant Staphylococcus aureus). Much like everyone else, time to time I think back and reflect to myself about my continual growth and progress. Really, my scientific career began as an intern just asking how to use a…
Staphylococcus aureus and Escherichia coli are common examples of multidrug resistant organisms, which causes severe infections in the community including hospitals [9]. Multidrug resistance makes the treatment of bacterial infections more difficult [9], and patients in the intensive care unit are vulnerable to these types of infections as resistance is common among this group [8]. Methicillin resistant Staphylococcus aureus is the first bacteria to play a major…
Bacterial identification plays an imperative role in pathology, enabling the diagnosis and treatment of many diseases abundant within the human population. Samples from biological tissues as well as artificial reservoirs, are frequently utilised for analysis in order to detect the presence of bacteria. These may be either normal flora, opportunistic pathogens or invasive microorganisms, from which analysis can deduce their role in disease states. The inaugural step in the process of bacterial…