Staining

The SH-SY5Y neuroblastoma cell line was procured from National Centre for Cell Sciences, India and maintained in (DMEM) (Sigma Aldrich, USA) and Ham 's F12 nutrient mixture (F12) (Sigma Aldrich, USA) in 1:1 ratio. The medium was supplemented with 2 mmol L-glutamine (HiMedia, India), 10% (v/v) heat inactivated fetal bovine serum (FBS) (Hyclone Laboratories, Logan, UT) and 1% antibiotics (penicillin/streptomycin). Cells were cultured at 37 ºC in a humidified CO2 ¬incubator containing 5% CO2 and…

3.1. Green photosynthesis of gold nanoparticles using aqueous extract of Baker’s yeast: One aim of the present study is the development of an improved method for derivatization of metabolites existed in aqueous extract of Baker’s yeast (Saccharomyces cerevisiae). This procedure facilitates identification of metabolites in Baker’s yeast extract and its utility for the synthesis of nanomaterials. The preparation of trimethylsilylenol ethers of metabolites was performed by treatment of the extract…

The first appointment consisted of the dental hygienist reviewing the patient's medical history with the patient and taking the patient's vital signs. The patient’s medical history revealed alcohol use, tobacco use, and no medications. However, the patient does take Mucinex-Sinus Max due to seasonal allergies. The dental implications associated with Mucinex-Sinus Max is xerostomia. Xerostomia affects the oral cavity in a negative way due to the lack of nutrients provided from saliva. The…

Neisseria meningitidis is the leading cause of bacterial meningitis in children and young adults. Extrameningeal manifestations of Neisseria meningitidis infection are uncommon in normal children and are a consequence of invasion of the bacterium into the blood stream. We describe a case of IMD with meningoencephalitis, septic arthritis, myositis and pleural effusion who developed secondary fever and prolonged hospitalization. Case report: A 10-year-old boy previously healthy was referred from…

floor with another kid next to me groaning,he looked younger than me.I got up, my knees and elbows were scraped and bruised,they were bleeding. Bright, red blood dripped down from my elbows and knees and hit the black top. Plop,Plop,Plop. It was staining the black top,but my elbows and knees did not hurt my forehead did and, I did not know why. Plop,Plop,Plop, went my blood again. The little boy got…

I strive to enhance the quality of human life. The human race is plagued with disease, disorders and societal plights that treatment with traditional medicine would cease to assuage the recurrent issues. For example, diabetes is currently one of the top health challenges in the United States. The influx of diabetic patients will not decrease with insulin treatment alone, one must alter their injurious diet. Physicians are expected to treat patients based on symptoms. The analysis is then capped…

Then, the samples were paraffin embedded, cut into thin sections, and mounted on microscopic slides. The slides were stained by a Modified Movat 's Pentachrome staining protocol. Three representative images, each of which covered approximately 1/3 of the media layer, were captured and used to obtain the average contents of the tissue components in this layer. Aortic aneurysms are attributed to medial degeneration…

Introduction For quite some time, researchers have noticed that the majority of teens recognize that they are overly-concerned with how much they eat. To put this into perspective, let’s look at some statistics: 40-60% of adolescents that are at normal-weight believe that they are overweight; nearly 50% of teens report smoking cigarettes to help them lose weight; and approximately 70% of girls admit that body shape is an important factor of their self esteem. [1] Shatkin. (2009). Common…

Phase 2 – Agarose Gel Electrophoresis 1. Obtain our four H, E, L, and P labeled micro tubes and place them on ice. 2. Set the micro pipet to 2.0 ul. 3. Transfer 2.0 ul blue dye to each of the four H, E, L, and P micro tubes. 4. Mix the four micro tubes by using this two-step method: * Place each tube briefly on the vortex to get the components to collect at the bottom of the micro tubes * Pulse spin the four micro tubes in the centrifuge to mix all of the components completely. 5.…

Cross Contamination of Brassica Napus INTRODUCTION Brassica Napus is a bright yellow flowering rapeseed that can be used to create biodiesel, animal feed and vegetable oils. As of 2009, over 90% of Brassica Napus was genetically modified to make it disease and drought resistant. The high rate of the genetic modification of this crop is due to the increased yields of the crops which in turn lead to higher economic returns for the farmers producing the Brassica Napus. (Beckie, 2011). Although…