Staining

anthracis, Yersinia pestis and others show anti-capsular antibodies in their serum. Capsule is impermeable to biological dyes routinely used, therefore they cannot be stained. Negative staining which colours the background is employed to demonstrate the capsule. India ink and Nigerosin are the two stains used for staining the background and later a simple stain like safranin or crystal violet is applied to stain the…

ultrasound before aspiration. There may be an obvious deformity or cause of the bursitis, but if radiographs come back normal then aspiration can be performed. Of the many tests on bursa fluid aspirate, Gram staining is one of the most precise (Baumbach et al., 2014). Although Gram staining is so accurate, it was used less than 50% of the time by physicians and orthopedic surgeons in Switzerland (Baumbach et al., 2013). Each type of bursitis, SB or NSB, can be treated conservatively or…

Then observed after staining with DAPI (4, 6-diamino-2phenylindole-2HCl, a fluorescent dye) which gives strong fluorescence when bounds DNA (Wicks BL et al, 1996). The other method for observantion of septa and DNA i. e. Calcofluor white-Sytox Green staining method wherer the slide washed three times with PBSand then fixed by using 1% Triton 100X in PBS. The agar section from the slide should…

INTRODUCTION Being able to identify an unknown specimen in microbiology is vital in the laboratory. The goal of this experiment was to isolate bacteria on a nutrient agar (NA) plate to obtain pure colonies of the unknown specimen. This study was done by applying the methods learned in Professor Sprenkles microbiology lab in determining the unknown bacterial growth present with use of pure culture colony morphology, cell morphology and arrangement, and Gram reaction. MATERIALS AND METHODS The…

Carvacrol modulates instability of xenobiotic metabolizing enzymes and downregulates the expressions of PCNA, MMP-2, and MMP-9 during diethylnitrosamine-induced hepatocarcinogenesis in rats Covered By: Kiauna Pinnock & Weschester Junior Nine researchers in the fields of biochemistry, nanoscience & technology and medical biotechnology from various research institutes and universities came together with an interest in Hepatocellular Carcinoma (HCC). HCC is one type of the malignant tumors that…

Abstract This paper talks about a clinical case study that describes the condition of a 60 year old man. I have identified that this patient presents symptoms of peritonitis. Peritonitis is the inflammation of the peritoneum and is usually caused by diverticulitis which this patient has, also the patient has a cloudy peritoneal fluid which indicates the sign of a bacterium called Bacteroides fragilis. This bacterium is obligately anaerobic and is typically found in the digestive tract of humans.…

centrifuge. After careful homogenization, duplicate smears were prepared and stained by ZN staining and AO FS techniques. Samples were processed by 5% NaOCl and USP techniques, and again, duplicate smears were prepared with deposit and stained by ZN staining and AO FS techniques. Deposit was cultured on LJ medium and BACTEC. Of 178 TB suspects, 36 (20.2%) were CP by either solid or liquid culture. Direct ZN staining detected 22 of 36 cases, and direct AO FS smears detected 26 of 36 cases. NaOCl…

Veneers are conservative cosmetic dentistry. Our ultra thin, porcelain veneers are designed to slide on top of your real teeth and require very little surface preparation. Other procedures that change the appearance of a tooth can involve grinding down a good deal of the tooth’s surface. Veneers strengthen worn teeth. This is especially important for older dental patients whose teeth are worn down from use. In general, porcelain veneers can also strengthen and protect the front of the…

because they penetrated and bound only to the structures in neuron cell bodies. There are two uses for the Nissl stain method when looked at through high and low magnification. Under low magnification you can see the brain structure by selectively staining groups of cell bodies whereas under high magnification, when using the Nissl stain, it allowed them to get a good indication of how many neural cell bodies were in an area because they could now count the number of stained dots that they saw…

I want to paint a picture for you. I want to depict a specific moment in time, in hopes that you may better understand my client. Imagine: you’re deep in the woods. Trees guard your every path. Fallen branches and torn grasses claw at your feet. Imagine your shivering body, clad in a thin nightgown, running from the demons that attempt to knock you to your knees. Now imagine how you got there. Imagine how you have reached such a belittling point that your only hope was to run as fast as you…