Staining

DISCUSSION: Pseudomonas aeruginosa was selected to study their antimicrobial activity due to the production of pyocyanin, an antimicrobial phenazine pigment. For this study different clinical specimen were collected and from these P. aeruginosa was isolated. To isolate good pyocyanin producing P. aeruginosa, repeated plating of all the isolates on cetrimide agar helped in final selection of the 5 isolates. To examine the antimicrobial activity of those isolates, in vitro inhibition by cross…

The purpose of the infections caused by gram-positive cocci lab exercise is to utilize different identification and diagnostic techniques to differentiate various groups of bacteria, which cause infection. Our primary goal is first to establish the presence of an infection; then, determine which particular pathogens, or groups of pathogens, are the underlying cause of the infection. In the clinic, infections manifesting from gram-positive cocci are a common and growing problem. Two genera in…

Introduction: Microbiology is the study of all living microorganisms like bacteria, fungi, protozoans…etc, and non-living microorganisms like viruses, viroids and prions. The significance of identifying an unknown microorganism is first to understand what type of organism it is and what structures it has to help figure out their potential benefit, danger or even neutrality to humans. Physicians benefit from this by identifying what their patients are suffering from and how to treat them. Also…

Three agar slants and three nutrient broths were inoculated with Staphylococcus epidermidis using aseptic techniques for transferring samples from broth, agar, and plate cultures. A sterile inoculating loop was used to transfer a loopful of culture from the culture to the sterile media. The lip of the culture tube and inoculated media tube was flamed before and after transfer. The inoculating loop was reserialized after transfer was complete. An agar deep transfer of S. epidermidis was also done…

Van Tran Microbiology Tuesday 5:00 May 5, 2015 Unknown Sample # 7 1. Introduction: Using biochemical tests are a good way to identify unknown bacteria from a sample. It is basically a similar to a trial and error method, but rather than using trial and error a person would be using positive and negative results. Using biochemical tests will help a person identify certain characteristics of the unknown bacteria through a series of chemical tests. Of course more than one biochemical tests…

The second test tube 11 was identified as a gram negative bacteria. The biochemical tests that I performed that were positive were catalase, glucose, SIM, indole, citrate, and urea. First, the catalase test has the presence of an enzyme in the test isolated detected using hydrogen peroxide. Bacteria possess catalase with a small number of bacteria; isolation is added to hydrogen peroxide when bubbles occur. When the bubbles occurred on gram positive bacterium it was a conformation of being…

Unknown #30 1a. Glucose fermented. 2a. Sulfur reduced. 3a. Lactose fermented…………………………………………Citrobacter freundii 3b. Lactose not fermented 4a. Sucrose fermented…………………………………….Proteus vulgaris 4b. Sucrose not fermented………………………………Proteus mirabilis 2b. Sulfur not reduced. 5a. Lactose fermented. 6a. Sucrose fermented. 7a. Ornithine decarboxylase present……Enterobacter aerogenes 7b. Ornithine decarboxylase not present…….Klebsiella pneumonia 6b. Sucrose not…

Soil houses a vast array of life including many different types of microbes. These microbes help contribute to the ecosystems in and around the soil. For the purpose of the study of this paper the ecosystem that the sample was collected from was the forest floor. The types of microbes that grow in specific soil samples depend on many things such as pH, salinity, temperature and the variant levels of different nutrients. Moisture levels of soil and energy sources are also large factors in…

Gathered from the data, it seems that commercial products are more effective than natural remedies; natural remedies made little if no contribution in inhibiting bacteria. This result however, does not signify that natural remedies are inadequate at inhibiting bacteria. It reflects that natural remedies are ineffective in inhibiting gram-positive bacteria. First natural product: Saliva for instance, is one of the body’s many defence mechanisms. Its main function is to prevent adhesion of…

The Enzyme Electrophoretic Separation of Proteins lab experiment will take place on March 7th, 2017, from 2:00pm to 5:00pm. It is important for students to follow lab safety guidelines in order to prevent any complications that may be a risk to one's health. For this experiment, students must wear their lab coat, goggles, and gloves at all times. All materials in this experiment should be handled with caution to avoid any damages. Students must keep hands away from face, eyes, and mouth, when…