Staining

The aim of this experiment is to identify and classify of an unknown bacterial isolate. Morphological and Biological test were preformed to identifiy the isolates characteristics. The following characteristics were tested: gram-stain, motility, morphology, hemolysis, lactose fermetation, mannitol fermentation, osmotolerance, aesculin hydrolysis, starch hydrolysis, gelatine hydrolysis, amino acid decarboxylation, lipid hydrolysis, lactose fermetation, casein digestion, catalase enzyme production,…

After obtaining my unknown organism the first thing I needed to do to get started was to do a gram stain. This would help me decide which kind of further testing would need to be done in order to help identify the organism that is causing gastrointestinal distress in Brown County. After doing a gram stain test on my unknown, I found that it was a gram negative organism. This prompted me to do some tests that are specifically for gram negative organisms. When I looked under the microscope I seen…

Purpose To identify an unknown microorganism by performing a series of biochemical tests on a pure bacterial culture. Materials and Methods 1. One test that was used to discover the identity of the given bacterial culture was the carbohydrate utilization tests. This specific set of tests involved two broths, namely phenol red lactose and phenol red sucrose, and a Durham tube placed upside down in the broth tubes. These broths can be broken down by fermentation processes,…

We first needed to collect all the proper materials to conduct the tests on our unknown bacterial sample. To begin the lab, we had to collect a nonpathogenic bacterial sample by swabbing an item or surface inside the biology building with a sterile cotton swab. Our lab group decided to swab a Sharpie permanent marker left at our lab station inside our lab room. We then opened a petri dish and smeared the swab across the surface of the agar several times. Once the bacteria was on the agar, we…

(Move to treatment??) Researchers have discovered that the fight against invading B. anthracis begins with the first infected cell. They found that the impacted macrophages will instantly communicate with other immune cells to warn them of the distress and develop a strategy in order to survive. The key signaling molecule that is involved in the survival strategy is adenosine triphosphate or ATP, which is a basic currency of energy transfer used by all organisms for various biochemical processes…

During the phase and process of the bacterial unknown project, vast amounts of test were performed in order to determine an unknown species of bacteria. Within the many procedures of identifying the unknown bacteria, I was first given a tube with the unknown bacteria, from which later on I started subcultures by streaking bacteria from the original tube onto a tryptic soy agar (TSA) slant. Throughout the process of identifying the unknown, new subcultures were produced in order to use the newest…

Gram + Cocci To identify the Gram + cocci I first performed a catalyst test which produced oxygen bubbles and indicated a positive catalase result. The next test I preformed was a mannitol salt test using a mannitol-salt agar plate and the results showed positive fermentation turning the plate yellow and also positive growth. This led me to conclusion that my unknown Gram + cocci was Staphylococcus aureus. Gram – Rod To identify the unknown Gram – rod I first performed a H2S production test…

My journey to the microscopic world of bacteria can be likened to a child exploring the world for the first time. As the child discovers the world, she also learns several survival skills along the way. In the same manner, all the knowledge I learned from Microbiology class and laboratory sessions regarding microbial world equipped me to identify bacterial unknowns from a clinical sample. The task was to successfully separate and accurately identify two bacteria of a specimen taken from an…

Introduction: Klebsiella pneumoniae is a part of the family Enterobacteriaceae. This genus is a gram negative and it has no flagella, which meant that this bacterium is not motile. However, it can move with the use of the current that is produced by the water. This bacterium has the ability to cause destructive change in a man or animals lungs if aspirated. In addition, this is also one of the most contacted pathogen in a Hospital Acquired Infection (HAI) or nosocomial infection. In this…

1n this report there is information about the safety rules which we have to follow in microbiology lab during experiments and various safety precautions we take in labs. A microbiology lab requires a unique environment & special practice. Then this report contains information about various kind culture media and there preparation which is essential for the culture plating detection of various microorganisms and bacteria There is information about various kinds culture plating techniques…