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    Polymerase Chain Reaction

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    Polymerase chain reaction (PCR) Generalities PCR is a method developed in 1985 by Kary Mullis and to obtain, by coupling a heat-resistant DNA polymerase and without cloning, amplification of a fragment of DNA known. Initially, DNA polymerase was isolated from a bacterium thermophilne (resistant to significant increases in temperature), as Thermus aquaticus (Taq polymerase). Currently, we use recombinant enzymes, whose; elaboration is easier and greater efficiency. The general principle of PCR is…

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    government could not even stop it. One of “The most important [organizations] was the 1933 Agricultural Adjustment Act (AAA), which aided farmers such as the Hendersons with a variety of programs ranging from drought relief to agricultural price stabilization” (Henderson 138). Many people admired President Theodore Roosevelt, because he arranged a very helpful organization where thousands of people got assistance throughout the Dust Bowl and the Great Depression. Price in the economy changed “By…

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    Animal well-being after hydrodynamic injections. In general, the mice tolerated very well the gene transfer procedure via hydrodynamic tail vein injection. All animals recovered from the hydrodynamic injections completely in 5 to 10 min without any signs of distress or problems. The blood creatinine levels did not raise from the pre-hydrodynamic injection baseline values, indicating that the putatively harsh gene transfer method was not harmful to the kidney function, or that one week was long…

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    Scotland’s Quest for Independence Since the 16th century Great Britain has had control over the economic welfare of Scotland. Some of the economic levers that Great Britain controls is currency, finances, oil sales and debt. This control over the years has caused a lot of friction and in 2014 the first vote for independent was made. This first referendum was voted down due to the people thinking that Great Britain provided a safe blanket and felt Scotland was too weak to stand on its own…

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    Evolution Of Managed Care

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    Evolution and Current Trends of Managed Care As the landscape of health care in America evolved, early care practices of the 1900’s gravitated toward a prepaid medical group practice, leading to a managed care approach to health care. Evolution continued leading to an expansion of health benefits, and various options of providers and services ultimately leading to a rise in health care cost that could not be overcome. This paper will discuss the forces of change for managed care, current…

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    However you may choose to refer to Henry Ford, unscrupulous robber baron, captain or industry, or visionary industrialist; his investment in Ford Motor Company’s human capital was revolutionary. Henry Ford knew if he could reduce labor time and costs in manufacturing, he could lower the price of the Model T; so he introduced the production assembly line, which reduced the time to build a Model T from twelve in a half hours to under six hours ("Henry Ford Changes the World, 1908," n.d.). To…

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    Connected Cars: Traditional Automobile Industry’s Strive for Relevance A connected car is an intelligent motor vehicle with in-built internet accessibility, which enables it to interact with a host of objects, such as – smartphones and wearables, city infrastructure such as smart parking and traffic signaling systems, surrounding motor vehicles, home/office automation devices, cloud infrastructure, etc. The traditional automotive industry is ripe for disruption over the next few years as the…

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    Ambiguity Of War

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    could imagine. The War on Terror is a war against attack from anyone. While supplying the world with weapons, they ultimately end up with boots on the ground; somewhere. An entire region has been demonized and the US has contributed to the de-stabilization of their governments. The War on Terror is an effort to contain the conflict within the Middle East but this has not succeeded. Terrorist organizations have expanded beyond the region into Europe and the United States. Attacking the US…

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    Is cooperative engagement only an idealistic strategy or could it be the first step to a very effective foreign policy that leads other nations towards the goal of international peace and security? Issues like, climate change, infectious diseases, or economic crisis are problems that can reach beyond a state’s borders, yet they have the potential to cripple a nation. The world is facing a new type of war, one that is different than other wars in the history books. The United States’ new foreign…

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    Capnography Monitoring

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    Implementation of Capnography Monitoring in Patients under Opioid Sedation: Evidence Based Practice Project Opioid-induced respiratory depression (OIRD) is the most dangerous and frightening of the opioid adverse effects (Pasero, 2012). Opioids slow down the central nervous system (CNS), which can lead to fatal respiratory depression, sedation, and coma (Zedler et al., 2015). The Joint Commission noted that 29% of opioid-related respiratory depression events were associated with improper patient…

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