Introduction: One of the most common mass spectrometry–based proteomic methodologies used to day is the bottom up protein identification. First, the protein has to be digested into peptides using a protease. Then, the peptide will be submitted to the URMC Proteomics Resource and Laboratory for analysis using LC-MS/MS tandem mass spectrometry. In this approach, the peptides will be separated and introduced into a mass spectrometer using reversed phase liquid chromatography (RC-LC). This…
Dehydration Synthesis process and the Macromolecules. I’ll start with the Macromolecules. These molecules are the four molecules all living things need to survive. The four molecules are Proteins, DNA, Carbohydrates, and Lipids. Each of these molecules is made of Carbon, Hydrogen, and Oxygen at the very least. Proteins have Nitrogen as an added element, and DNA has Phosphate and Nitrogen. The base structure of each molecule has a carbon skeleton. This means that structures have Carbon as the…
of proteins in this fraction. For fraction 4, a higher concentration of imidazole was used to wash the solution, the gel showed the band migrated and stopped at 38 kDa. For fraction 5, highest concentration of imidazole was used and the migration stopped at 38 kDa as well. It represented the presence of purified protein in the fraction by showing only one band at the site, which indicated the protein was purified. The expected SpHTS protein was around 40 kDa. In the gel, the purified protein was…
structure and function of cellular components can be used as evidence for evolution. The two fields that contribute to molecular evolution research are molecular biology and biochemistry. Molecular biology is the study of cellular components such as proteins, carbohydrates, nucleic acids, and lipids. Biochemistry is the study of the chemical processes in organisms. These two fields of science act as evidence because they show that organisms…
our project was to simulate the digestion of carbohydrates, lipids, and proteins. Using a certain medication called pancreatin we tested its effects on how it was able to break down these macromolecules. pancreatin is a mixture of pancreatic enzymes that help to aid in digestion; the enzymes that are present in this mixture include amylase (breaks down carbohydrates), lipase(breaks down lipids), and protease (breaks down proteins). We had set up trials for these enzymes to break down their…
The aim of this paper was model the amino acid intake of broiler chickens for optimum economic in different scenarios with MC how to example. The second objective was described in software R the scripts for modeling the optimum amino acid intake for broiler. In addition, we considered the price of whole birds and DL-methionine cost according to Economic scenario. The dilution technique was used to allow formulates the experimental diets with the same balance of amino acids for all treatments…
receptor-mediated endocytosis, unlike the other two processes is a selective process; this consists of cell proteins in pits on the surface of the cell acting as receptors which bind with molecules and the cell will only absorb certain cells if they are bound to a particular receptor.…
Gene Therapy & Cancer Cells Gene therapy is often defined as a treatment that uses genes to cure or prevent diseases. Genes, DNA, and protein are all involved with each other, therefore, in many immune diseases, if one fails your whole immune system could eventually fail. This treatment is relatively new; hence it is still a pretty risky experimentation since it involves getting genes into cancer cells. Gene Therapy is often involved in serious cancer cell treatments; its main purpose is to try…
Sickle Cell Disease is a blood disorder that’s inherited and affects almost 100,00 folks in the United States . Red blood cells contain hemoglobin which is a protein that carries oxygen in the blood . Normal red blood cells are flexible and round which allows body . The sickle cell disease causes red blood cells to form a cresent shaped red blood cells to break away easily causing anemia . Instead of the normal 120 days the…
(there are other types but the ones mainly discussed in protein synthesis only involved these two). The DNA and RNA are partners that work together to make protein in protein synthesis. DNA is like the instruction manual to making the protein. The first step involves making the mRNA through a process called transcription. In transcription, a small portion of DNA is "copied" through coding to make the mRNA. Now entering the next stage of protein synthesis, the mRNA leaves the nucleus to go to…