Solutions

The purpose of this experiment is to learn how to prepare albumin from egg white and to understand the basics of protein fractionation using ammonium sulfate. Ovalbumin is the major protein of egg white and is fractionated by means of ammonium sulfate precipitation. The properties of the solvent system will dissolve the protein and greatly impact solubility. In total, there are four major factors that affect protein solubility: ionic strength, pH, temperature, and dielectric constant of solvents…

Emily Melendez January 31, 2017 BSC2010 Enzyme I: pH and Temperature Lab Report Introduction: (2008, November 11). Without Enzyme, Biological Reaction Essential To Life Takes 2.3 Billion Years. Retrieved January 31, 2017. The basic concept that is being explored are about enzymes. Enzymes are proteins that are known for being sparkplugs in a chemical reaction. Enzymes have a shape and for them to ‘spark’ they must have the proper substrate for it to work U. This does not change the enzyme’s…

The neutral pH and room temperature tubes produced the most foam. This is because the enzyme is most reactive at these points. pH 7 is like water being added to the solution, the other pH levels are too acidic or too basic for the enzyme. Room temperature involves the enzyme in its natural state. When the pH level is too acidic/basic or when the temperature is too cold/hot the enzyme begins to denature, or breakdown…

take if you were to break a beaker with a pH 9 solution. First, once a spill occurs, ensure everyone (especially the lab instructor/advisor) around the area or in the lab knows about the occurrence. Second, it would be best to make sure that you have not been contaminated with the chemical, meaning that you should take care of yourself by washing the affected areas. Third, once your hands are dry, you should then make an effort to neutralize the solution of pH 9 as close as possible to pH 7 and…

Throughout this acid, base and buffer lab, this will allow each individual to gain an enhanced sense of knowledge of how these different aqueous solutions work as a buffer system or how they are unable to perform as buffer system mixed with two solutions together. This can be identified through the colour change to measure pH behaviour in several aqueous solutions, therefore the different factors that will cause the change of pH. This will gain understanding of how this relates to the biological…

Proteins are known as the building blocks of cells in all living organisms. Many hormones are made up of proteins and the digestive system, immune system and blood replies on proteins. Therefore, protein is an essential part of healthy diet, and integral to development of the body. The purpose of performing this lab is to analyzing the activity of TEM-1 B-lactamase and how it reacts with high temperatures, chaotropic salts, detergents and pH values. INTRODUCTION: Primary protein structure is…

To determine optimal pH value for the hydrolysis of p-NPG by leukocyte GCase, we examined effects of variable pH (4.0-5.5) at a constant substrate concentration (5mM). Figure 1 shows the pH activity curves of leukocyte GCase assayed in the presence and absence of 6 mg per ml Sodium taurocholate. In the absence of the pure sodium taurocholate, the activity in the range of pH 4.0-4.8 was considerably lower. However, in the presence of pure Sodium taurocholate, leukocyte GCase was optimally active…

objective of our company. They also stated that they weren’t able to focus on the menu because they are trying to prevent people from cutting in front of them in line. The result of these problems is a tense and stressful environment. Possible Solutions: One solution would be to…

Since acetic acid is considered a weak acid (strong within the scale of weak acids), there is a range at which the acetic acid and acetate will form a stable buffer. Using the Henderson-Hasselbalch equation for buffers, it’s possible to calculate the pH based on the ratio of acid to conjugate base, or calculate the ratio given the pH and pKa value. Finding charge on a protonated amine: The pKa value given for the amino group on any amino acid specifically refers to the equilibrium between the…

For this catalase experiment it was decided that the group was to keep the concentration of catalase and hydrogen peroxide the same and only change the level of pH buffer throughout the experiments conducted. This was done to observe the effect different levels of pH buffers have on the breakdown of hydrogen peroxide and the volume of oxygen released. The results from the majority of experiments conducted reflect that though they are in consensus with the hypothesis that the volume of oxygen…