stirring bar. The pH of the solution was then checked using the pH meter and it was brought down to the desired pH of 4.8 using a couple of drops of glacial acetic acid. Then it was poured into a 500-mL graduated cylinder and brought up to a volume of 1000 milliliters using distilled water. The buffer was poured into a labeled glass bottle and stored in the fridge. Another sodium acetate buffer was made using the same…
Optimisation studies were carried out on the basis of varying initial concentration of Chitosan and Xanthan, and varying pH of Chitosan solution. The results obtained were interesting. Unstable capsules were formed with the following combination of Xanthan and Chitosan concentration. The Chitosan solution pH was kept constant at 5. The above table gives the inference that when Xanthan concentration is less than or equal to 0.5% or Chitosan concentration is less than or equal to 0.5%, the…
First, a buffered solution of acetic acid and sodium acetate was made by combing 15 mL of .5 M acetic acid, 15 mL of deionized water, and 1.020 grams of sodium acetate. A solution of .25 M NaOH was then prepared using 25 mL of .5 Ml NaOH and 25 mL of deionized water. The burette was then conditioned with 10 mL of deionized water twice and twice with…
system (concentration of acetic acid and sodium acetate) increases. Tables 1 and 2 show values for the solutions (acetic acid and sodium acetate) that were made in week 1 of the experiment. They also state the precise concentration of the final solution based on the precise volume and concentration of the initial solution. Table 2 also shows the mass of sodium acetate added for the 0.60 M solution. Tables 3 and 4 show the results for week 2 of the experiment. They state the moles of NaOH or HCl…
The 1992 book Ordinary Men: Reserve Police Battalion 101 and the Final Solution in Poland, written by Christopher R. Browning, seeks to understand the police officers behind the blitzkrieg against Polish Jews during the German military offensive of 1942. Rather than focus on the liquidation of major ghettos in Warsaw and in Lodz, this study focuses on the smaller towns and villages that included significant Jewish populations in Central Poland. By examining indictments and judgements from legal…
Systems As humans, we constantly need to regulate our bodily pH. This regulation process is called homeostasis, and is controlled by the hypothalamus and pituitary gland by a buffering system. A buffer solution is used to maintain the pH at a safe acidity. “A buffer solution is an aqueous solution consisting of a mixture of a weak acid and its conjugate base or a weak base and its conjugate acid” (Boundless, 2010) Equation 1 – Generic Buffer Equation HA (aq) + H2O (l) --> H3O+(aq) +…
The Tris Base was dissolved each time in a 125mL Erlenmeyer flask with 80mL of water. Deionized water was added to each buffer to obtain a 100mL buffer solution. The pH was recorded by a pH meter for buffer 6 and buffer 8 The effect of added acid or base was measured under various conditions. Solution mixtures were created that included: water with 0.1M HCl, water with 0.1M NaOH, 0.1M HCl with buffer 6, 0.1M HCl with buffer 8, 0.1M NaOH with buffer 6, and 0.1M NaOH with buffer…
followed by the activation carboxylate groups on Si and Au electrode in 75mM N-(3-(Dimethylamino)propyl-N’-(ethylcarbodiimide hydrochloride) and 15mM N-hydroxysulfosuccinimide sodium salt in 50mM buffer solution for about 1h. The antibody coated electrodes were created by immersing for 1h into a solution of 50µg/mL antibody and 50mM Phosphate-buffered saline at pH 7.3, which results in forming of amide bonds to amine groups on the protein surface, and then it is immersed into 0.1% BSA for 1h in…
OVERVIEW Four ablations will be done in ex vivo bovine for each flow rate. There will be three different flow rates: 0 mL/min, 860 mL/min, and 1000 mL/min. These flow rate were chosen based on hepatic flow rates of humans in the supine position (Brown et al. 1989; Leen et al. 1992). Each ablation will be performed at 40 watts for 2 minutes for a total of 12 ablations. The average area of a 30ºC and 50 ºC contour at the end of the ablation will be used to characterize the size of the ablation…
for reference) which can be seen as an effect of the specific pH on the catalase enzyme. This identified trend can also be supported by the raw data values of the control test tubes as it demonstrated that the hydrogen peroxide and the pH buffer solution did not affect the boiling chips (which did not contain the enzyme catalase) and it was from the presence of catalase within the potato discs which caused the resulting froth…