Serum total protein

MUST FIND OUT WHY THIS VEIN IS USED The animal is put into a crush. Raise the tail vertically with one hand until it is horizontal with the ground and wipe the underside of the tail to remove any manure present. The tail is then held in one hand such that the ventral surface is accessible. The ventral surface of the tail is cleaned with a swab to remove faecal material. A needle is then inserted perpendicular to the skin surface on the midline between (approximately) the third and fourth coccygeal vertebrae, (Zubair Shabbir et al., (2013)) QUESTION 2 The Packed cell volume or PVC is an estimate of the percentage of cells within the blood (Reeder, (2009)). Total protein (TP) = A total serum protein test measures the total amount of protein in the blood. It also measures the amounts of two major groups of proteins in the blood: albumin and globulin (WebMD, 2016). Fibrinogen is a soluble glycoprotein synthesized by the liver. . It is used to diagnose and monitor a variety of inflammatory conditions in the horse (Gerros,…

shoot fresh weight (g) and lengths (cm) were measured from respective plants on each day of sampling. For dry weights of roots and shoots, the samples were dried at 72 ºC for 72 h. Samples for RNA extraction, cDNA synthesis and qRT-PCR were immediately frozen at −80 ºC. Chlorophyll content of the leaf tissue was determined by using Chlorophyll Meter (SPAD 500 Plus, Minolta, Japan) (Markwell 1999). Third or fourth leaf from each plant/replicate was used for total protein contents measurement by…

Separation of plasma proteins by molecular exclusion chromatography Sebastian Prisacariu 140013826 Introduction The components of blood is broken down into 3 parts. This breakdown is consisted of: Plasma which is a yellowish fluid that helps transport blood cells around the body and contains a small supply off proteins, platelets which are blood clotting agent, red blood cells which is a carry for oxygen and remover off carbon dioxide and white cells which is the bodies defence from…

contamination protein), where the amounts of exosome protein were still uncertain. To solve this issue, we tested 10% of each tag-labeled sample using LC-MS/MS before mixing, and obtained the amount of exosome protein by a label-free method. High-abundance contamination proteins including all forms of IgG and FN were deleted from the list of identified proteins. The remaining proteins were assumed to be derived from exosomes. Then, all peptide intensities of exosome proteins were log2…

For larger peptides having molecular weight of 1.5 – 10 kDa, aqueous protein precipitation is done using TFA ot TCA upto 10%, followed by evaporation or solid phase extraction to remove the acid. PEGylated proteins of larger sizes, with molecular weight of 40-50 kDa, possess a unique hydrophobicity which allows their precipitation. The PPT step denatures the proteins and removes matrix effect causing componenets. The precipitated proteins are then digested using trypsin and the analysed using…

Does Milk Fat Percentage Affect Curd Mass? Purpose The purpose of this lab was to determine if the percentage of fat in milk would have an effect on the mass of the curd. Background Information Milk is about 3.3% total protein and these proteins contain all nine essential amino acids. Milk proteins can be categorized into two groups, the casein proteins and the serum proteins. Casein proteins contain a phosphorous and will coagulate at a pH level of about 4.6; serum proteins do not contain…

The SH-SY5Y neuroblastoma cell line was procured from National Centre for Cell Sciences, India and maintained in (DMEM) (Sigma Aldrich, USA) and Ham 's F12 nutrient mixture (F12) (Sigma Aldrich, USA) in 1:1 ratio. The medium was supplemented with 2 mmol L-glutamine (HiMedia, India), 10% (v/v) heat inactivated fetal bovine serum (FBS) (Hyclone Laboratories, Logan, UT) and 1% antibiotics (penicillin/streptomycin). Cells were cultured at 37 ºC in a humidified CO2 ¬incubator containing 5% CO2 and…

Inhibitor-1(PAI-1)4G/5G polymorphism with oxidative stress markers Malondialdehyde (MDA) and High sensitivity C-reactive protein (hs-CRP), fibrinolysis marker PAI-1and lipid profiles. Subject and Methods: Blood was drawn and DNA extracted from 90 subjects (46 cases and 44 controls). The 4G/5G polymorphism of PAI-1 was amplified using specific primers. Amplified products were visualized by staining with Ethidium bromide after electrophoresis in agarose. Risk factors PAI-1, MDA, hs-CRP and lipid…

 COMPOSITION OF MILK Milk is nearly complete natural food on the earth. It contains water, fat, protein, lactose and minerals as major constituents. The proportions of these constituents vary with species of the animal. For example, Mare’s milk contains only about 1.6% fat, while Reindeer’s milk contain 22.5% fat. Apart from this, the composition also varies with several other factors, viz., the breed of animal, the feeding pattern, season, lactation period, age of animal, environment, etc..…

Introduction So what is GGT? GGT stands for Gamma-glutamyl Transferase, it is found on the cell surface that contribute to the extracellular catabolism of glutathione (GSH). The most GGT serum is derived from the liver, but it is also produced in many other tissues. GGT is primarily carried with lipoproteins and albumin in serum. The levels of GGT is determined by various factors such as alcohol intake, body fat content, plasma lipid/ lipoproteins and glucose levels, and also various medications…