Serum albumin

certain antigens based on their epitope, which is usually five to eight amino acids long on the surface of the protein. Western blot is used to visualize the proteins that will respond to specific antiserum after we separate the proteins. Then place the serums against nitrocellulose…

Observe Ligand Protein Interaction using Gel Filtration (Phenol Red Binding to Serum Albumin) Introduction: Serum albumin is often known as blood albumin, it is the most abundant plasma protein in humans and other mammals. Serum albumin is essential for maintaining the osmotic pressure that is needed for distribution of body fluids between body tissues and intravascular compartments. It is also a carrier protein by non specifically binding several biomolecules. Ligand is a molecule can bind…

Observe Ligand-Protein Interaction using Gel Filtration Chromatography (Phenol Red Binding to Bovine Serum Albumin) Introduction: Serum albumin is often known as blood albumin, and is the most abundant protein in humans and other animal plasma. Serum albumin is also known as a carrier protein by non-specifically binding several biomolecules. It is essential for maintaining the osmotic pressure that is needed for body fluids distribution between body tissues and intravascular compartments.…

The technique used in this experiment was serum protein electrophoresis to determine the levels of proteins in cow serum. The objective of this experiment is to perform a gel electrophoresis of cow serum by agarose gel electrophoresis and to interpret the banding patterns produced by the migration of the serum. Electrophoresis is the shift of ions in an electric field in which the protein moves in the gel based on size as well as in electric charge. The electrophoretic mobility of small…

First lane is the protein ladder which is used as a reference and to estimate the size of different proteins. Lane 1 in the green box contains cow serum which shows high mobility since it dyes all the way to the bottom with different bands through the gel. Lane 2 contained one dark band at the bottom of the gel. Lane 3 shows o dark band with low mobility through the gel. Lane 4 shows one band stained with medium mobility through the gel. Sample proteins 1-4 were separated through gel…

3. Measurement of glycated albumin As mentioned before, the measured albumin is considered a reliable tool to supplement other methods of measuring blood glucose, such as HbA1c and standard assays for glycemic control and diagnosis diabetes [30]. The HbA1c lifespan is estimated to be about 90 to 120 days. Thus, it is known as an indicator for the assessment of long-term glycemic control [17, 31]. However, the level of HbA1c is not suitable for the assessment of the patient’s status in some…

Cytochrome C, Myoglobin, Albumin and Hemoglobin(proteins) based off of their net charges. Materials: • Rabbit hemoglobin • Serum albumin(cow) • Myoglobin • Cytochrome C • Microtubes • Tape • Micropipets • Agarose gel • Buffer • Gel chamber • Test tubes • Test tube holders • Gloves • Macropipets Properties of Proteins: Protein Color Isoelectric Point Net Change at pH 8.6 Cytochrome C Orange 10.2 Positive Myoglobin Brown-red 7.2 Negative Hemoglobin Red 6.8 Negative Serum Albumin Blue 4.8 Very…

guidelines. Intravenous albumin (1.5 g/kg within 6 hours of spontaneous bacterial peritonitis (SBP) diagnosis and 1 g/kg on day 3) should be given along with intravenous antibiotics. Multiple studies have shown IV albumin effective in decreasing the incidence of renal dysfunction from 33% to 10% and mortality from 29% to 10% and is especially beneficial for patients with baseline serum creatinine ≥ 1 mg/dL, blood urea nitrogen (BUN) ≥ 30 mg/dL.1 Patient BB has a baseline serum creatinine level…

from infection. The plasma makes up about 55% of the overall volume of blood and is mainly made up of water which contains important proteins like albumin which maintains fluid balance (Nall, 2013) and globulin which serves as antibodies and helps with transport (Clinn,…

Inflammation is an essential part and highly regulated response to injury that promotes healing and provides protection against infection. Inflammation is frequently painful and hot, which can be easily controlled by nonsteroidal anti-inflammatory drugs. In 2012, 98 million NSAIDs were prescribed in the U.S. (“Treating Osteoarthritis and Pain” www.consumerreports.com) NSAIDs are commonly used to treat pain and reduce fever and inflammation by reducing the amount of prostaglandins the body makes.…