SDS-PAGE

electrophoresis. The second process consists of another electrophoresis but at a right angle to the direction to the first electrophoresis that was done (1). SDS is added in this second process and separates the proteins based on their size forming the one-dimensional SDS-PAGE (1). The first gel is soaked in SDS and situated on the edge of the SDS polyacrylamide-gel slab where the polypeptide chains move towards to and form a spot (1). Thus the second-dimension of two-dimensional…

The hypothesis of the experiment was that the molecular weight of N-acetyl-β-D-hexosaminidase would be around 28,000 kDa but to determine the molecular weight the concept of SDS-PAGE had to be understood. The movement of any charged particle through an electric field is determined by its net charge, its molecular radius and the magnitude of the applied field (7). Being that natively folded proteins are not molecular weight dependent, the proteins would be traveling at different speeds in an…

Summary The aim of this study was to examine the level of similarity between scores on two Holland instruments; the Self-Directed Search (SDS) and the Holland Personality Mosaic (HPM) and to provide information validating the HPM instrument (Miller et al., 2008). The study attempts to measure the convergent validity of the HPM by comparing the results of it to the SDS results using the lachan Index. Convergent validity takes place when one instrument is compared to another instrument that…

Self-Directed Search Form R: An Assessment and Reflection The Self-Directed Search (SDS) Form R provided a comprehensive introduction for an individual to survey what profession to pursue. As a student, I was afforded the opportunity to simulate the function as both test administrator and client of this instrument. There were three important lessons I have taken away from this exercise: first, progression through the administration process remained tedious, but was needed in order to aid in…

The aim of this report is to examine alcohol consumption patterns of a population in New Zealand comparing male and female amount and frequency of alcohol consumption. New Zealand has a long history of alcohol abuse starting in the 1830s when Europeans started settling in New Zealand (McEwan, Campbell, & Swain, 2010). Prior to the arrival of the Europeans there was no alcohol drunk in New Zealand and Reverend W. J. Williams said in 1930 "The white man and the whisky bottle came together to…

the probability of the behavior" (Page 321) This can be linked to the ability for children to develop verbal learning. With stimulus control for example, if a child see's a human being is walking with their dog they with start to see some differences in features and hear the human call this strange being a dog. Then through stimulus generalization which is "the tendency for an operant response to be emitted in the presence of a stimulus that is similar to an SD" (Page 322) the child will be…

America to get involved. One of the groups fighting for equality and democratic participation were the Students for a Democratic Society, or SDS. The SDS wrote a political manifesto where they proposed a system to encourage participation called The Port Huron Statement, or PHS The Port Huron Statement was written by Tom Hayden and 58 other college students in the SDS, on June 15, 1962. The main purpose of the PHS was to express the dissatisfaction many college students in the 1960’s felt towards…

Preparation for Cell Lysis Obtain the mammalian expression vector for homo sapien ESR2 from Addgene. Assemble the vector to code for the proteins snail, slug and twist. Transfect HEK293 cells with the modified plasmids in petri dishes. Allow the cells to grow for 1-2 days so they will express the proteins. Transfer the culture medium to a centrifuge tube to separate it from the cells. Add 1mL of phosphate buffered saline (PBS) to the cells and swirl to mix. Separate the PBS from the cells and…

Figure 2 shows the SDS-PAGE of the fraction number, which is the X-axis of Figure 1. In figure 2, a constant level of protein must be used through each lane of the SDS, in each step. The sample enzyme is placed in the gel, between every lane. A current is then run through the gel. The number of bands, in each lane, decreases with increasing…

How Ferritin was first isolated and how it is isolated now The protein ferritin is formed from two classes of subunits, H and L, in a ratio which vary in different cell types. The H-type are subunits associated with rapid uptake of iron and are predominantly from the red blood cells. Subunits that take up iron much slower are designated L-types. A third type subunit, M, is found in amphibians and is similar to the H subunit. The M-types are predominant in the liver. Once synthesized, ferritin…