Scanning electron microscope

Cellular Theory & Microscopes Robert Hooke Robert Hooke was an English scientist credited most famously for the creation of Hooke’s Law (a principle of physics that states that the force needed to extend or compress a spring by some distance is proportional to that distance) in 1660 and the discovery of plant cells using a compound microscope in 1663. The compound microscope uses two or more lenses, which are connected by a hollow tube. The top lens (called the eyepiece) is the one that people…

A light microscope works like a telescope using glass lenses to reflect light, which magnifies images which are naked to the human eye such as cells and dust. The lens closest to your eye is called the eyepiece lens. This usually has a magnification of x10. Objective lenses are lenses of several different strengths which magnify the image. The stage is the place where the microscopic slide sits. This is held in place by the clips. The light source (can be a mirror or a bulb) projects light onto…

semiconductor material. This P-N junction is a connection of a p-doped (electron-hole-doped) semiconductor material and n-doped (electron-doped) semiconductor material. When electric current through this junction is present, the electrons participating in the electric current are of an elevated energy state and propagate in the conduction energy band. The conduction energy band exists at a higher energy than the valence band, which is where electrons occupy the valence states of an atom. In an…

There was an extra control slide so that the microscope could be adjusted without harming the samples. The cover slip was placed on top and nail polish was added to the sides of the cover slip to prevent the sample from drying out. With the 40x lens of the Zeiss Axiovision Widefield Fluorescence Microscope, images of the cells were captured. The two channels that were used were DAPI and DIC. DAPI had an exposure time of 960…

Discussion Microscope is an optical instrument that have magnifying lens which is used for inspecting objects that is too small to be seen or to be seen distinctly and in details by the unaided eye. Microscope also is an optical instrument that keep observe specimen in resolution and magnitude way. However, there are some microbes such as viruses that cannot be observe by certain microscope and this microbes has to be observe using electronic microscope which has more magnification power. In…

A 12-well tissue culture plate was used to carry out the LIVE/DEAD Assay. Well “A” contained the untreated HepG2 cells. Well “B” contained camptothecin, which is used as a control for killing the HepG2 cells instead of the use of plumbagin. Well “C” contained the compound Epigallocatechin Gallate. Well “D” remained empty, because a vehicle treatment is not needed for this experiment. Each well contained 1 ml of HepG2 cells. A negative and positive control where both set up in the 12-well tissue…

Lab Five: Microscope and Simple Staining Introduction A microscope is a tool to examine enlarges image of small objects that are hard to study with the naked eyes. The light microscope, which was used in this lab experiment, is an instrument with two lenses and various knobs to focus the image. We will learn about the proper way to use and handle the microscope. Microorganisms are measured with an ocular micrometer, which is inserted into one of the microscope eyepiece. Micrometer serves as a…

For the purpose of this assignment, I researched an article titled, “The Role of DJ-1 in the Pathogenesis of Endometriosis” by Priyanka Rai and Sisinthy Shivajj. The article discusses many components in which are relevant to the disease, endometriosis. Endometriosis is a disease among women in which causes infertility and severe pelvic pain along with other health concerns. It is seen to occur within 10% of woman whom are of a reproductive or sexually-active age. It is due to the occurrence…

An inverted fluorescence microscope is an inverted compound light microscope equipped with modules to internally separate the different wavelengths of light, and direct them to respective detection channels. Its main components include a light source, an objective, a filter cube and a detection unit, which can be an eyepiece or a camera (Fig.1). Filter cube is a component which separates a fluorescence microscope from a light microscope. It consists of one excitation filter, one emission filter…

The slide used during the Microscope Lab was a wet-mount slide which was used in order to view the specimens from the microscope. A wet-mount is a method for preparing specimens to view using a microscope. A myriad of specimens look better when placed in a drop of water on the microscope slide. The water supports the sample and fills the space between the coverslip and the slide. This allows light to pass easily through the slide, the sample, and the coverslip. Scientists use the wet-mount…