Caffeine Impact on Goldfish Metabolism Introduction Metabolism is a process that enable organisms to maintain their life. Metabolism can be measured in numerous parameters. Within this experiment in order to measure the metabolism rate by determined the level of dissolved oxygen. Goldfish is an example of poikilotherm that are organisms that are influenced by the environment surrounding them. Thus, the manipulation of surrounding can influence the metabolism rate due them being ectotherms.…
out of a sample bones, I have separated them artiodactyla from rodentia by species, then obtained a numbered count for how many there were of each. Thus, the NISP. Quantifying taxa by NISP is: simple, time effective and can be replicated in the future by researchers to obtain…
alternate E. coli strain. Methods: Each individual received either E. coli A or B strain of pKN800 plasmid DNA. The plasmid was purified and isolated, then cut with PstI restriction enzyme to create a sample of PstI-cut and uncut pKN800 plasmid DNA and to distinguish orientation A from B. These samples were loaded onto a 0.8% agarose gel for electrophoresis. Both cut and uncut plasmid DNA were transformed into E. coli DH5α. Finally, the transformed DNA was plated on LB and LB with ampicillin…
24 hours in a refrigerator. After 24 hours each plant sample was filtered through 4 layers of cheese cloth and then filtered through filter paper twice. The filter paper that was used was Whatman Qualitative Grade 1 Plain Circles, and multiple filters were used during the process. The samples was filtered through 0.45 um Millipore filters (EMD Millipore Stericup Sterile vacuum filter units). Once all filtered processes were complete the samples was covered with parafilm and store in -4degree C…
The sample size for the study was 8 students which is a very small sample size and cannot be used to form any sort of conclusion as the data collected was very small and not suggestive of an entire population. Second weakness of the study was the excessive involvement of the researchers in the…
Agarose gel electrophoresis of DNA is used to seperate mixtures of protein and nucleic acid fragments. Molecules tend to differ in size, charge, and mobility. The objective of this procedure is to predict the distance of DNA migrating towards the positive electrode in the electrophoresis chamber. Many scientists use agarose gel electrophoresis to examine the DNA of criminal suspects. The first step in this procedure is to prepare a buffer containing 1% of the liquid agarose gel. Once the gel…
land decreases as the marsh reaches towards the marsh front, and figurative – that data taken from sample sites at the marsh such as pH, amount of organic matter deposited within the soil, and amounts of specific sediments deposited within the soil has a linear pattern as the marsh extends to the shore. This report will examine elevation, soil pH, water salinity, and soil texture data from unbiased sample sites in hopes of finding a relationship to support the hypothesis of this…
(DCPD). Amorphous calcium phosphates, prepared by precipitation from supersaturated solutions, can also react to form apatitic cements since they are thermodynamic unstable with respect to HA and have a setting reaction more independent of particle size. Calcium phosphate cement containing an antibiotic can be used for filling bone defects and to ensure local antibiotherapy. Therefore, in the present chapter proposal, cement paste were prepared by combining cement liquids comprised of Na2HPO4…
It provides many statistics about homelessness covering from migrants to aboriginals. Everything about homelessness is covered in this article. The sample size, valid source, statements supported by facts and improvements to read the data will be covered in the following essay. This article about homelessness in Australia clearly states the sample size in the subtitle. The evidence is “For every 10,000 people, 50 are homeless, and more than 43,500 homeless people are under 25” (Melissa Davey…
presence indicates that the water may be contaminated by human or animal wastes”. Some bacteria break down organic matter and play an important ecological role, other support in the human metabolism and usually they are classified under their shape, size, nutrient type, chemical activities, pathogens or non- pathogens, and physical condition for growth. • Protozoa, are “unicellular complex microorganisms” with no cell walls that nurtures on…