This was conducted on the second group of infected mice. Blood samples from the mice were taken each day. The blood was analyzed with another Surveyor assay to measure CCR5 gene disruption and then western blotting to compare HIV-1 viral RNA loads. A positive result that supports the hypothesis of the CCR5 ZFN-disruption would be bands on the Surveyor nuclease assay that travel further down the gel. This indicates that the mismatch was present and the nuclease correctly excised it from…
Parents should not be allowed to play God or Nature to choose a child's gender. Women are choosing the gender of their children for reasons such as genetic diseases, family balancing, or just wanting the choice. According to Sureau , " In the prevention of X-linked diseases, e.g. Duchenne myopathy, haemophilia, there are the following choices: (i) pre-natal diagnosis, followed by medical termination if the fetus is male or termination which is restricted to affected male fetuses; (ii)…
Transforming Genetic Material To E. Coli Using Plasmids Proteins are created within the body through a process known as central dogma. This process is the transcription of DNA to RNA and the translation of RNA to proteins. The type of protein created relies on what type of messenger RNA or mRNA that was produced during the transcription of DNA. In our experiment, we looked at the specific LacZ gene that codes for beta-galactosidase, which is a protein/enzyme that can break down lactose into its…
Genetic mutations are the source of numerous hereditary diseases. This has been discovered by the comparison of two DNA sequences with bioformatic software. Two sequences are aligned and compared in order to locate mutations in the DNA (Module 4 Protocol,1). Escherichia coli aka E. coli was used as the test subject in this lab because it employs lac operons while transcribing mRNA. An operon is a unit of linked genes that regulates genes in charge of protein synthesis. A lac operon requires…
Gas Exchange + Transport Pulmonary circulation: circulation from heart to lungs Systemic circulation: circulation from lungs to organs Gas exchange is the movement of oxygen from the lungs to the blood and the movement of carbon dioxide from the blood to the lungs. This exchange occurs in the capillaries, which are microscopic vessels in the lungs. In humans, nutrients and oxygen are transferred through the bloodstream. Cells can’t rely solely on diffusion, so this transport system is important…
contact, and through sexual intercourse. The Zika virus is thought to be the leading cause of microcephaly, but the article talks about why it is the leading cause and why it isn't. Major findings for why Zika virus causes microcephaly is that virus RNA has been found in placenta, amniotic fluid, and brain tissues of stillborn babies with microcephaly. According to the CDC one woman who have traveled to a foreign country with Zika, her child was born with microcephaly.Recent studies have begun…
I chose this source National Archives which lead to Link of Our Document, because I think it best represents the science component of “The Birthmark”. Author of my short story, Nathaniel Hawthorne grow up in 1804 and published “The Birthmark” in 1843. In 1880, Thomas Jefferson published his patent for the light bulb. It was patented on January 20, 1880, the light bulb is a scientific advancement and/or improvement that lead to society as we know it now. The light bulb is a a glass bulb inserted…
As a senior, my short-term academic goal is to do my best and pass all of my five AP exams. English is my second language, so I struggle with writing essays, but I have been practicing writing essays in order to achieve a score of four in my AP English Literature Exam. My short-term personal goal is to be better at time managing. I have always been punctual, but lack time managing skills. There are many sleepless days in my junior years due to my desire to finish the work on time, but I was…
Huntington’s Disease Huntington’s disease is a fatal genetic disease disorder that makes progressive breakdown of nerve cells in the brain. It literally deteriorates a person's physical and mental abilities, it has no cure. Huntington's disease is known as the quintessential family disease. People affected by this disease can have trouble walking and speaking. Huntington’s Disease was first discovered as an inherited disease in 1872. Huntington’s disease is a dominant gene. Since it is a…
of those who are chronically infected will develop liver cirrhosis or liver cancer. The diagnosis for HCV is if the antibody test if reactive, an additional blood test is needed to determine if a person is currently infected with Hepatitis C. If the RNA test comes back positive then they do have the virus. There is no vaccine for hepatitis C but some things you can do to prevent getting this virus is practice healthy hygiene; including surgical hand preparation, hand washing and the use of…