RNA

Introduction: DNA, RNA and Proteins, these are three types of very large molecules essential for every living organism, for their biological functioning. Each molecule, DNA, RNA and Protein, play a vital role in living organisms, without them no life could survive. Let’s understand functionality one by one: • DNA consists of encoded instructions which are necessary to maintain, assemble and reproduce. • Proteins play important role in movement, photosynthesis, vision etc. • RNA is used to make…

Virus Taxonomy Order: Unassigned Family: Bunyaviridae Genus: Phlebovirus Species: Toscana virus talk.ictvonline.org Structure and genome The toscana virus (TOSV) is a negative-single stranded RNA virus, it is spherical in shape, it has an enveloped surrounding its capsid, and it has a diameter of 80–120 nm. Its genome is partitioned into three segments, termed (large)L, (medium)M and (small)S. With L about 6,400 nucleotides in length, M about 4,200 nucleotides in…

a. Introduction Lujo Hemorrhagic Fever is caused by a bi-segmented negative RNA virus that is one of several viruses known to cause viral hemorrhagic fever. The Lujo virus is part of the Arenaviridae family, which consist of several viruses categorized into two groups. The Lujo virus is categorized in the “Old World” group, which signifies that the virus in question was found in the Eastern Hemisphere, specifically the continent of Africa. The majority of the arenaviruses categorized in the…

RNA polymerase attaches itself to a template of DNA and then go into base pairing, synthesizes mRNA or messenger RNA. This is called transcription, as the DNA code being transcribed into mRNA code. RNA replaces Thymine for Uracil during base pairing. 4. mRNA leaves the nucleus and enters the cytoplasm this goo like part of the cell where ribosomes can…

Photothermal therapy would support the nano-carriers with the RNA by creating a pathway through the cell to the nucleus. In which the nanomedicine will introduce foreign RNA to modify the genetics while the reactivity of the heat from the P.T therapy would enhance the strength of the nucleotide. There are two methods for this drug delivery. One takes place above 50…

the DNA unwinds and unzips leaving the base pairs exposed. Helicase is used in this reaction- the enzyme breaks the hydrogen bonds between the complementary bases. Activated RNA nucleotides then bind with the complementary base pairs exposed where A binds with U, G binds with C- the enzyme which catalyses the reaction is RNA Polymerase. The final mRNA strand which is produced is complementary to the original DNA strand. The mRNA is free to leave the nucleus through the nuclear pore and attach…

now unwound in the area that is needed so the RNA polymerase is able to help line up the nucleotides to create a complementary strand of the mRNA. Between the strands there are hydrogen bonds which are then separated apart. One of the strands is then able to be used as a template strand. Onto this template strand these is base pairing which is the DNA cytosine joining to the RNA guanine, DNA guanine joining to the RNA cytosine, DNA thymine joining to RNA adenine and DNA…

Explain how in one organism codon usage and tRNA abundance might be connected. Codon brings the genetic material that could translate to protein. Each codon contains triplet nucleotide, and each of them code for an amino acid, two different nucleotide sequences can encode the identical protein. There is some special codon that code for the initiation and termination of translate. The most common start codon is AUG, and the stop codon are UAG, UGA, and UAA. tRNA has a tertiary structure, and…

a template for RNAs capable of recognizing viral DNA within a cell and as a means to bind the endonuclease CRISPR associated protein 9 (Cas9). These complexes allow the enzyme to be shuttled to the target DNA to perform a cut on one or both strands of the foreign DNA in either of the enzyme’s two active sites. Recently, scientists have repurposed this bacterial defense mechanism as a genome editing utility to cut, shuttle, or bind desired molecules anywhere within DNA as custom RNA harnesses the…

Function of potyvirus proteins The Potyviruses genome contains one open reading frame (ORF) which is translated as a large polyprotein (between 340k and 368k), that is cleaved into 10 functional proteins (Riechmann et al., 1992) such as: Protein P1, HC-Pro, P3 protein, CI, NIa , NIb, 6K1, 6K2, VPg. The potyviral P1 protein is a serine protease that cleaves at its own C-terminus (Verchot et al., 1991). This is the most divergent potyviral protein in size (30-63 kDa) and sequence except protease…