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    It is also conceivable that, at lower pH, depurination can occur via protonation of the purine nucleobase at both N1 and N7. The presence of the 2′-OH has a significant effect on the nucleoside’s susceptibility to depurination. For example, guanosine and adenosine are more resistant to depurination compared to deoxyadenosine and deoxyguanosine. Deoxyadenosine itself depurinates 1200 times faster than adenosine (York, 1981). Interestingly, N-acyl-protected purine nucleosides (particularly…

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    Synthesis Of ATG-ACT-TGA

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    with the ATG codon. Most biological activities are carried out by proteins. In order to have the DNA sequence that reads ATG-ACT-CAT-GAG-TGA to be translated into the RNA to build a protein there has to be proteins that are not the same to be able to perform this function. The DNA also needs to be changed into the RNA and the RNA has to be changed into a protein. In order for the cell to have a proper function the proteins that are necessary need to at the time that is proper be synthesized.…

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    Alkaline phosphatase (AP) is a homodimeric enzyme complex that is commonly found in a wide range of organisms, from bacteria to all tissues of the human body. AP is a zinc metalloenzyme (1), in which metal ions play a key role in the regulation of catalytic activities and stabilization of enzyme-substrate complex. As proposed by Gettins and Coleman using NMR studies (10), each active site of AP comprises of three metal binding sites, which acknowledged as M1, M2, and M3. Two zinc ions bind to…

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    Under oxidative conditions, certain nucleobases (for example, adenine and cytosine) can form N-oxides. Also, the C8 position of guanosine is vulnerable to hydrolytic attack under either strongly acidic or strongly alkaline conditions. The 5,6 double bond of pyrimidine nucleosides also reacts with halogens and halohydrins to give the corresponding addition products (Shabarova and Bogdanov, 1994). Selected examples of the side reactions that occur during oligonucleotide synthesis are given below.…

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    Leroy et al. (2000) used 4 microsatellite primers to characterise Brassica oleracea accessions. Among the 136 reproducible fragments generated, 25 (18.4%) fragments were common for all Brassica, 27 (19.9%) were unique and 84 (61.7%) were phylogenetically informative. Flannery et al. (2006) assessed polymorphisms in Brassica, Arabidopsis, Camelina, Raphanus and Sinapis using 10 plastid SSR primer sets. Eight loci were polymorphic, and separated the individuals of Brassicaceae into taxon-specific…

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    6F4Z1003- Genetics, Adaptation and Diversity Practical Report Section 1- Calculations and Pedigrees 1. In DNA extraction the proteins absorb light at 260nm and 280nm, especially the aromatic acids. 2. 250µg to nm, 250 x 1000 = 250,000ng. 3. 200ng/ml = 0.2µg/ml. 150µg/ml = 150,000µg/ml 250,000pg/µl = 250µg/ml 1mg/ml = 1000µg/ml ● 150,00µg/ml - HIGHEST CONCENTRATION OF DNA ● 1000µg/ml ● 250µg/ml ● 0.2µg/ml - LOWEST CONCENTRATION OF DNA 4. Sodium dodecyl sulphate is used as a…

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    Icosahedral Structures

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    around the surface of a sphere. The corners of the structure are what we refer to as vertices, and each icosahedron comprises 12. These structures display a 2-3-5 symmetry, where there is 2-fold symmetry that rotates through the middle of the edges, 3-fold symmetry that rotates through the middle of each face, and 5-fold symmetry that rotates through the middle of the corners. These triangles are built from identical subunits, which in this cause prevent gaps from occurring between subunits,…

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    Chrality Research Paper

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    Chirality is a geometric property of some molecules and ions. A chiral molecule/ion is non- superimposable on its mirror image. The presence of an asymmetric carbon atom is one of several structural features that induce chirality in organic and inorganic molecules. Many biologically active molecules are chiral, including the naturally occurring amino acids (the building blocks of proteins) and sugars. In biological systems, most of these compounds are of the same chirality: most amino acids are…

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    Why Mutations Are Random

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    Mutations are random. There are several reasons for mutations to occur. This essay will discuss what is a mutation, furthermore, it will examine different types of mutations that can occur, and it will inform you about how helpful and harmful mutations really are. Mutation is a change that occurs in the deoxyribonucleic acid sequence, gene or structure, due to mistakes that occur when the DNA is transcripted or because of environmental changes. Mutation occurs when there is a mistake in the…

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    Organelle Failure Nucleus: The nucleus of a cell is, without doubt, one of the most important parts of the cell. The nucleus instructs every single part of the cell what to do, and what the different organelles need to do. The nucleus includes chromatin (protein and DNA). It also contains Nucleolus (nucleoli is plural). These nucleoli are responsible for ribosome production. If the nucleus were to suddenly malfunction, this would mean that the cell would have no directions, and nothing to…

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