RNA world hypothesis

Explain how in one organism codon usage and tRNA abundance might be connected. Codon brings the genetic material that could translate to protein. Each codon contains triplet nucleotide, and each of them code for an amino acid, two different nucleotide sequences can encode the identical protein. There is some special codon that code for the initiation and termination of translate. The most common start codon is AUG, and the stop codon are UAG, UGA, and UAA. tRNA has a tertiary structure, and…

CRISPR Cas9 CRISPR Cas9 is a gene editing tool that can be used to edit, delete, and change parts of the genome. What makes CRISPR Cas9 different from other gene editing techniques such as Zinc Finger Nuclease (ZFN) or Transcription Activator-Like Effector Nucleases (TALEN) is its targeting efficiency ability. For example, ZFNs and TALENs could only reach targeting efficiencies from 1% to 50% in human cells. On the other hand, CRISPR Cas9 had a much higher rate at greater than 70% in Zebrafish…

now unwound in the area that is needed so the RNA polymerase is able to help line up the nucleotides to create a complementary strand of the mRNA. Between the strands there are hydrogen bonds which are then separated apart. One of the strands is then able to be used as a template strand. Onto this template strand these is base pairing which is the DNA cytosine joining to the RNA guanine, DNA guanine joining to the RNA cytosine, DNA thymine joining to RNA adenine and DNA…

referred to as ZIKV, has become a global health epidemic. The World Health Organization declared a Public Health Emergency of International Concern earlier this year due to the increase of areas affected by Zika. Originating in the Zika forest of Uganda, ZIKV was predominantly found in areas of Africa and Asia. However, there have recently been reports of more than 36 countries with cases of the virus. (Chen, 1), (CDC) ZIKV is a single stranded RNA virus that was originally found in a rhesus…

Genetic disorders may affect millions of people every few seconds around the world, and it’s our job to understand how they work or occur in order to try and prevent them or cause them from getting worse. With how far research has come over the years, we now know how genetic disorders occur. They occur from something called mutations, which I am sure mostly everyone has heard about. Most people just don’t understand exactly how mutations work, occur, or how they cause millions of different…

A Comparison of Protein Synthesis between Eukaryotes and Prokaryotes The different fundamental organisation of genetic material and organelles between eukaryotes and prokaryotes leads to dissimilar processes of protein synthesis. The larger eukaryotes have a more intricate pathway associated with protein synthesis, due to both the biochemical structure of organelles and enzymes, and also the transport of genetic material within cell. In this essay I hope to explore the differences of gene…

Figure 22. Relative mRNA expression in INS-1E cells after different chemicals treatment. Cells were treated with DHT, TCS and TCC for 48 h before RNA extraction for activity determination. Expression levels of the AR, pdx1, GK and Ins2 were determined by RT-QPCR and mRNA expression levels were normalized with TFIIB (A-D). DMSO treated samples were considered as control. Control samples were set as one and results are shown as mean ± S.E.M of duplicate samples from three independent experiments.…

diversity among transposition elements and mainly classified into two groups according to their movement mechanisms. Class I is retro-transposons which use a RNA intermediate in transposition mechanism and Class II is DNA transposons remaining as DNA throughout transposition [4]. Retro transposons first transcribe themselves in RNA and then RNA is copied to DNA again by reverse transcriptase which is generally encoded by mobile elements. Copied DNA is inserted into target site. The main class of…

DNA gives cells the potential for specific behavior, but this potential will only be realized if the gene is expressed. A gene is expressed if the DNA is transcribed into RNA and then converted into a protein. These proteins can have many different effects on the cell, such as metabolism or neurotransmission. We are now able to tell which specific genes are expressed at a particular time. Gene expression is uncommon, however, with no transcription and only the potential for a behavior as a…

The role of polymerase chain reaction(PCR) and Gene therapy in medicine. PCR was developed by Kary Mullis in the 1980s. It is a method in which a single copy of DNA is amplified. Using this technique which has a specific sequences ,millions of copies of DNA can be produced. In this process, many other conditions including several enzymes and primers are involved. This application of PCR extent from research to the commercial sector. Denaturation:- The reaction mixture is heated up to…