RNA polymerase

monounsaturated oleic acid (18:1). Polyunsaturated fatty acids are undesirable in biodiesel because they confer oxidative instability, but greater energy (J.C. Sedbrook, et al, 2014). Targeting gene function reduction of FAD2 in Thlaspi arvense using RNA interference method, lowered linoleic/linoleic acid content and increased oleic acid content in seed oil. We are hoping…

Basic Biology The documents I attached include a lab report, worksheets, notes and one exam. My most memorable experiences in biology were in the lab. I got to test the effectiveness of lactase drops when exposed to solutions containing different pH or temperatures. My results indicated that the lactase enzyme had the optimal catalytic activity at temperatures similar to those found in the human stomach; however, the low pH in the stomach–ranging from 2 to 5– rendered the drops ineffective…

Current molecular biology is being done by analyzing damaged DNA and by applying RNA techniques to forensics. Research is now being done with establishing useful markers on the Y chromosome. These markers on the Y chromosome would be especially helpful in sexual assault cases. A very intersting part of all the new Y chromosome research…

Theory, RNA World, etc.). Life chemically first arose from inorganic matter due to the environment created by hydrothermal vents, the production of RNA, and clay hydrogels. Primarily, the environment created by hydrothermal vents could have led to…

2.5. Morphological Identification/Evaluations of Entomopathogenic Fungi The six isolates of fungal entomopathogens were provided by Department of Entomology, Penn State USA. Preliminary morphological identifications of fungal entomopathogens such as color of colony, mycelial characteristics and spores from all six isolates were done at microscopic level. 2.6. Molecular Characterizations of Entomopathogenic Fungi Cultures of Metarhizium fungus were grown in nutrient broth (Bacto, Australia) in…

about because RNA was formed. This occurred because organic molecules on Earth were able to form ribonucleotides from the assistance of a clay catalyst. Eventually, these ribonucleotides formed RNA, which, through time, began to fold and replicate itself. As replication got faster, RNA's started to attract amino acids and hold them together. Bonds started forming, and proteins were able to be produced. As more years passed, a lipid membrane enclosed the structure to protect the RNA and proteins,…

While this may be the case, the antibody (IgM) cross- reactivity with other flaviviruses can be a problem when trying to come up with a precise diagnosis that is specific to Zika virus. In most cases, many tests often confirm the presence of the virus RNA in the blood of the patient or any other body fluids such as semen, saliva, or…

PCR is a common practice used to amplify a certain region of DNA. In this experiment, PCR was used to amplify the 16S ribosomal RNA gene of the bacteria. This locus was used because it is one that evolves slowly in bacteria. A Taq polymerase is used for this PCR because of the temperatures the reaction goes through. The16S rRNA primers used in this PCR, are complimentary to sequences conserved in all bacteria and will target sites of…

level shows as transcript per million (TPM) and the highest isoforms expressed in brain (blue) is ENST00000453943, heart (orange) is ENST00000312726 and liver (green) is ENST00000391865. The sashimi plot shows the quantitative visualisation of the RNA-seq read alignment of brain, heart and liver together with the gene expression shows in FPKM value (Figure 4.12C). The junctions show by the arcs that align from one exon to another exon. The visualisation of read alignment with the gene model on…

It is well known that all organisms require at least some water to survive, even the most hardy creature on the planet earth, the tardigrade, Hypsibius dujardini, which can survive near complete dehydration, exposure to extreme radiation, vacuum, temperature and pressure, must be rejuvenated with water if it is to resume life after near complete desiccation (Gabriel, et al. 2007). H. dujardini, famous for surviving some of the harshest conditions of any organism we know of, is a perfect example…