RNA interference

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  • PMT Genes

    Results Design of the RNAi vector The PMT gene family is well conserved among the Solanales. There are five genes in the PMT gene family (PMT1 to PMT5) which differ in the exon 1 sequence due to the insertion of a variable number of 33 nucleotide repeats. This makes the first exon as an ideal region for the design of gene specific RNAi trigger sequence (Fig 1A). Full-length sequences are available for four PMT genes (PMT1 to PMT4) whereas only the sequence of exon1 is known for PMT5 which…

    Words: 1625 - Pages: 7
  • Gene Regulation Research Paper

    GENE REGULATION: Gene regulation is the method of limiting the genes in the cell’s DNA, which are expressed. It is utilize to form a functional manufacture for an example protein. Various cells in a multi cellular organism might express very difficult group of genes. Although, they consist the similar DNA. Human having over ten thousands of genes in their genome. Cells are expressed the all genes. Even an organism as simple as bacterium must carefully regulate gene expression. Ensuring that the…

    Words: 1325 - Pages: 6
  • Nidulans

    Aspergillus nidulans: One of the lesser known pathogen of the aspergilli group, A. nidulans is a model filamentous fungus widely used for studying eukaryotic cell biology (Galagan et al., 2005). A. nidulans possesses a phospholipid-hydrolyzing novel cPLA2 protein, PlaA, which shows maximum similarity to mammalian-type cPLA2 proteins (α, β, γ) (Hong et al., 2005). Like the three isoforms of human cPLA2 proteins, A. nidulans PlaA also consists of two separate catalytic domainsA and B, and…

    Words: 1807 - Pages: 8
  • Protein Synthesis Paper

    This paper is going to describe the replication of DNA and RNA and the processes of transcription and translation of protein synthesis. What is DNA? DNA is a nucleic acid that carries the genetic information in cells and some viruses, consisting of two long chains of nucleotides twisted into a double helix and joined by hydrogen bonds between the complementary bases adenine and thymine or cytosine and guanine. DNA sequences are replicated by the cell prior to cell division and may include genes,…

    Words: 400 - Pages: 2
  • Hutchinson Gilford Progeria Research Paper

    Progeria, otherwise known as Hutchinson-Gilford Progeria syndrome , is a rare disorder that causes children to age eight times faster than a normal person. This disease affect only 350 kids today. It was discovered by Jonathan Hutchinson in England in 1886 and was first called Progeria by Hastings Gilford. It was then named Hutchinson-Gilford Progeria syndrome. The name Progeria is taken from a Greek word that means "prematurely old". It is an autosomal recessive disorder, meaning that an…

    Words: 758 - Pages: 4
  • Unit 18 Genetics: Questions And Answers

    Unit 18 - Genetics (Assignment 1) Task 1. Deoxyribonucleic acid (DNA for short) is genetic material that carry’s the majority of information for development growth and many other functions. DNA is located in the nucleus of a cell. DNA has a double helix structure and is formed by a series of bases these are Adenine , Cytosine , Guanine and Thymine. These bases have a basic pattern when forming DNA. Adenine will only join to Thymine when in DNA and Cytosine will only join to Guanine when in…

    Words: 782 - Pages: 4
  • Essay On Macromolecules

    When we think “polymer”, we should not limit ourselves to its biological applications; polymers can do so much more. Polymers are the “recipe for life.” We find them everywhere we look. For example, turn to your friend. What is he or she made of? The answer is polymers. Polymers make up essential molecules in our bodies, such as proteins or nucleic acids. They may be microscopic, but their functions in our bodies are important. Background Information There is so much more to being…

    Words: 462 - Pages: 2
  • Three Main Reasons For Memory Loss Of Memory Retrieval

    cue-depending forgetting, state-dependent learning, interference, transfer of training, and repression/suppression. To define these briefly, cue-dependent forgetting requires some sort of retrieval cue to be able to remember the information. State-dependent learning means that you must be in the same state in order to remember. Interference can have retroactive interference (new information causes you to forget the older information) and proactive interference (learning too many things at once…

    Words: 816 - Pages: 4
  • Difference Between Human And Chimpanzee

    A human being and a chimpanzee are quite similar not because of physical features but because of the DNA, they inherit and what makes these species so unique to one another. All living cells contain DNA, which is the basic unit for growing, reproducing, and surviving. DNA, Deoxyribose Nucleic Acid is comprised of 4 different types of bases; adenine, thymine, guanine, and cytosine that are proteins. These 4 bases rely on each other because of adenine pairs up with thymine, while cytosine…

    Words: 1214 - Pages: 5
  • Pancreatic Adenosquamous Carcinoma Case Study 1

    pancreatic ASC, I will isolate total RNA from UPF1 mutant cell lines and perform RNA sequencing. To establish whether the upregulated genes are direct targets of NMD, I will compare this data with RNA isolated from cells where UPF1 is downregulated using short hairpin RNAs. To validate the top targets from RNA sequencing experiments, I will perform qPCR and northern blotting. To examine whether these candidates are also altered in pancreatic ASC, I will analyze the RNA levels of top targets in…

    Words: 1047 - Pages: 4
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