Ribosomal RNA

Introduction: DNA, RNA and Proteins, these are three types of very large molecules essential for every living organism, for their biological functioning. Each molecule, DNA, RNA and Protein, play a vital role in living organisms, without them no life could survive. Let’s understand functionality one by one: • DNA consists of encoded instructions which are necessary to maintain, assemble and reproduce. • Proteins play important role in movement, photosynthesis, vision etc. • RNA is used to make…

Photothermal therapy would support the nano-carriers with the RNA by creating a pathway through the cell to the nucleus. In which the nanomedicine will introduce foreign RNA to modify the genetics while the reactivity of the heat from the P.T therapy would enhance the strength of the nucleotide. There are two methods for this drug delivery. One takes place above 50…

in creating important structures found in our body such as deoxyribonucleic acid, more commonly referred to as DNA, and ribonucleic acid or RNA. (Erster, Lecture 4 Chapter 5) Ribonucleic acid is then categorized into two types of RNA: mRNA and tRNA (there are other types but the ones mainly discussed in protein synthesis only involved these two). The DNA and RNA are partners that work together to make protein in protein synthesis. DNA is like the instruction manual to making the protein. The…

Fundamentals of Biochemistry in the Biotech Industry Maple Syrup Urine Disease: A Sweet Scented Killer Maple Syrup Urine Disease (MSUD) is a rare, potentially deadly disorder where the body cannot break down the three branched- chained amino acids (BCCA): leucine, isoleucine and valine. These amino acids are used to build proteins and when they are not being used they can be broken down and recycled in the cell. The complex that breaks these amino acids down is called BCKD (branched-chain…

DNA is the element of making an organism. It contains genetic information that can be applied to all kinds of functions required for living. DNA is a sequence of nucleotides which can be transcript to mRNA. When mRNA is formed, it can later on translate to tRNA where codon could be found. Codon is a set of nucleotide in tRNA which is directly related to the formation of amino acid. The combination of codon determines the characteristic of the amino acid. In other words, to make amino acids,…

a. Introduction Lujo Hemorrhagic Fever is caused by a bi-segmented negative RNA virus that is one of several viruses known to cause viral hemorrhagic fever. The Lujo virus is part of the Arenaviridae family, which consist of several viruses categorized into two groups. The Lujo virus is categorized in the “Old World” group, which signifies that the virus in question was found in the Eastern Hemisphere, specifically the continent of Africa. The majority of the arenaviruses categorized in the…

DNA purification: To get pure DNA to use, a silica membrane was used to collect the long DNA strands. We started by adding 5 volumes of PB buffer to 1 volume of each PCR reaction. We put column and the silica wafers into tubs and pipetted the DNA samples onto the wafers. We centrifuged the tubes for a minute and disposed of the waste. After that we pipetted 0.75 ml of buffer PE to the samples and spun it for another minute then disposed of the waste. The columns were added to the tubes and spun…

This is accomplished by adding salt and ethanol to a solution containing DNA or RNA. In the presence of salt (in particular, monovalent cations such as sodium ions (Na+)), ethanol efficiently precipitates nucleic acids. The purified precipitate can be collected by centrifugation, and then suspended in a volume of choice. PROTOCOL:…

sequencing and visualized by gel electrophoresis. It also can be cloned into plasmids. PCR is used in many fields of medicine, molecular biology research, medical diagnostics, and ecology. It is fast and inexpensive technique used to amplify DNA and RNA fragments by 107 times. The polymerase chain reaction was…

GENE REGULATION: Gene regulation is the method of limiting the genes in the cell’s DNA, which are expressed. It is utilize to form a functional manufacture for an example protein. Various cells in a multi cellular organism might express very difficult group of genes. Although, they consist the similar DNA. Human having over ten thousands of genes in their genome. Cells are expressed the all genes. Even an organism as simple as bacterium must carefully regulate gene expression. Ensuring that the…