Reverse transcriptase

Importance of Microbes Introduction It was long ago when Man realized that, along with animals and plants, he was also accompanied by some other organisms as well, and though which remained hidden from his eyes. Those organisms were unveiled by Anton Van Leeuwenhoek, who first saw the tiny organisms which accompanied Man through the lenses of microscope. Later it was found out that microorganisms inhabited each and every surface of earth, on man himself, or even inside him, resided under the…

EIA Equids obtain different diseases and some have cures while others do not. Equine infectious anemia is one of those without a cure. Many studies have been conducted on this topic so more can be known about this disease. Most of this can be avoided when purchasing an equid for personal use by talking to the previous owner. Equids are any type of animal that has hooves and is related to the horse including donkeys and zebras. This paper takes a look at equine infectious anemia and will examine…

It is hypothesized that motor proteins, dynein and kinesin control the transport of melanosomes into melanocyte dendrites as it is the net sum of cytoskeletal forces on an organelle that regulate intracellular transport. Dynein typically walk towards the minus-end of a microtubule in retrograde transport while kinesin move towards the plus-end in anterograde transport in neurons. The purpose of the article was to determine the role and effect of cytoplasmic dynein in melanosome transport in…

Ebola: The Next Pandemic Throughout a lifetime, everyone encounters an illness at one point or another. Most people know someone who has had a serious medical condition such as cancer, an autoimmune disease, or a debilitating illness. However, many of these conditions take months or years to develop. Death from a medical condition normally doesn’t happen with in a few days. The Ebola virus is an exception. Soon after this virus invades the human body, hemorrhagic manifestations begin. This…

1). In a total reaction volume of 30µl, RNA (3µg) dNTP’s (10mM) antisense primers (5picomoles) and DTT (5mM) were added and denatured at 65oC for 5minutes. Then, the samples were plunged into ice. To this reaction mix, 50 Units of reverse transcriptase (MuMLV) and reverse transcription assay buffer (5X) was added and incubated at 30ºC for 5 minutes. First strand cDNA was synthesized at 42ºC for 60minutes. Thereafter, the abundance of the PMT members and HCT was studied using gene specific sense…

Influenza is a highly contagious respiratory infection caused by the influenza virus. The illness can range from mild to severe, and can infect people of all ages, races, genders, and sizes. Generally, the illness is not life threatening in most people, but certain groups are at higher risk of requiring hospitalization or even dying from influenza. These at-risk groups of people include young children, the elderly, and people with compromised immune systems. The CDC estimated that there were “25…

Plasmids are extra chromosomal DNA within some bacteria that exist as circular structures. Although they are not essential for survival of the bacterium, they often do confer many environment advantages such as antibiotic resistance. One bacteria of interest is E. coli, a gram-negative organism that is part of normal human gut flora. Some strains of E. coli are pathologic to humans, however some strains are beneficial. For example, one strain produces vitamin K, an essential nutrient. In this…

Restriction enzymes are used to manipulate DNA sequences to create recombinant DNA by first cutting up the foreign DNA in order to protect the bacteria cell against invading DNA from other organisms. The enzyme is very specific when it comes to identifying a specific DNA sequence. When the enzyme identifies the specific DNA sequence it cuts both DNA strands at specific points at the restriction site. Lastly, the DNA ligase joins the DNA from two different sources and produces a recombinant DNA…

The CCR5-Δ32 Allele: A Natural Resistance to HIV Virus Human immunodeficiency virus, most commonly known as HIV, is an acquired virus that attacks the immune system of the host and eventually leads to acquired immunodeficiency syndrome otherwise known as AIDS. HIV is a relatively new disease in human populations, AIDS was first observed in homosexual males in 1981 (Herron et al., 2014, p. 2). Later, in 1983, soon after the discovery of AIDS, scientists identified HIV virus as being responsible…

Table: 9 FT- IR analysis of methanol extract of M. concanensis leaves S. No Peak values Intensity range Intensity Group assignment Functional groups 1 3972.43 0.0086 Unknown 2 3859.33 0.0412 Unknown 3 3805.79 0.0733 Unknown 4 3396.46 0.9280 Medium N-H stretch Amides, alcohols and amines 5 2987.43 0.2450 Medium C-H stretch Alkanes and acids 6 2946.27 0.2810 Medium C-H stretch Alkanes and acids 7 2912.38 0.2040 Medium C-H stretch Alkanes 8 2834.49 0.3170 Weak C-H stretch Aldehydes and…